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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F05%3A00003153%21RIV08-MSM-25310___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Byly sledovány problémy při imunomagnetické separaci listerií a byly porovnávány dva druhy imunomagnetických částic(Dynal BiotechxQuantum Magnetics). Pro zjištění nespecifických reakcí během IMS byly použity čisté kultury Listeria innocua, Arthrobacter spp., Bacillus subtilis, Citrobacter braakii, Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae and Staphylococcus aureus. Ve většině případů vykazovaly nespecifické reakce gram pozitivní mikroorganismy, zejména St. aureus a Arthrobacter spp. Nespecifické reakce během IMS byly ověřeny na uměle inokulovaných potravinových vzorcích, ve kterých vykazovaly gram pozitivní bakterie nejvyšší procento záchytu. Byl rovněž studován vliv promývání pufry. Problems occurring during the immunomagnetic separation (IMS) of Listeria using immunomagnetic particles Dynabeadsâ anti-Listeria (Dynal Biotech, Norway) were specified. Characteristics of these particles were compared with anti-Listeria spp. magnetite particles (Quantum Magnetics, USA). Pure cultures of Listeria innocua, Arthrobacter spp., Bacillus subtilis, Citrobacter braakii, Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae and Staphylococcus aureus were used to evaluate non-specific reactions during IMS. Gram-positive microogranisms, especially Staphylococcus aureus and Arthrobacter spp. of the initial were found to be responsible for non-specific reactions in most cases. The capacity of Dynabeadsâ anti-Listeria particles was determined to be about 10 % pure cultures of Listeria spp., after 10 min of incubation. Non-specific reactions during IMS of Listeria were examined on the artificially inoculated food samples in which Gram-positive bacteria showed the highest percentage of capt Problems occurring during the immunomagnetic separation (IMS) of Listeria using immunomagnetic particles Dynabeadsâ anti-Listeria (Dynal Biotech, Norway) were specified. Characteristics of these particles were compared with anti-Listeria spp. magnetite particles (Quantum Magnetics, USA). Pure cultures of Listeria innocua, Arthrobacter spp., Bacillus subtilis, Citrobacter braakii, Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae and Staphylococcus aureus were used to evaluate non-specific reactions during IMS. Gram-positive microogranisms, especially Staphylococcus aureus and Arthrobacter spp. of the initial were found to be responsible for non-specific reactions in most cases. The capacity of Dynabeadsâ anti-Listeria particles was determined to be about 10 % pure cultures of Listeria spp., after 10 min of incubation. Non-specific reactions during IMS of Listeria were examined on the artificially inoculated food samples in which Gram-positive bacteria showed the highest percentage of capt
dcterms:title
Nespecifické reakce při imunomagnetické separaci listerií Non-specific Reactions during Immunomagnetic Separation of Listeria Non-specific Reactions during Immunomagnetic Separation of Listeria
skos:prefLabel
Non-specific Reactions during Immunomagnetic Separation of Listeria Non-specific Reactions during Immunomagnetic Separation of Listeria Nespecifické reakce při imunomagnetické separaci listerií
skos:notation
RIV/00216275:25310/05:00003153!RIV08-MSM-25310___
n3:strany
397-401
n3:aktivita
n13:Z n13:P
n3:aktivity
P(GA203/05/2106), Z(MSM0021627502)
n3:cisloPeriodika
4
n3:dodaniDat
n14:2008
n3:domaciTvurceVysledku
n10:3777111 n10:7035411 n10:7862105
n3:druhVysledku
n15:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
533063
n3:idVysledku
RIV/00216275:25310/05:00003153
n3:jazykVysledku
n16:eng
n3:klicovaSlova
immunomagnetic separation; immunomagnetic particles; non-specific reactions; Listeria
n3:klicoveSlovo
n8:immunomagnetic%20particles n8:Listeria n8:non-specific%20reactions n8:immunomagnetic%20separation
n3:kodStatuVydavatele
HR - Chorvatská republika
n3:kontrolniKodProRIV
[486A3A8E19A4]
n3:nazevZdroje
Food Technology and Biotechnology
n3:obor
n19:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n17:GA203%2F05%2F2106
n3:rokUplatneniVysledku
n14:2005
n3:svazekPeriodika
43
n3:tvurceVysledku
Vytřasová, Jarmila Pejchalová, Marcela Červenka, Libor Zachová, Iveta Štěpánková, Jana
n3:zamer
n4:MSM0021627502
s:issn
1330-9862
s:numberOfPages
5
n9:organizacniJednotka
25310