This HTML5 document contains 55 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n3http://localhost/temp/predkladatel/
n19http://linked.opendata.cz/resource/domain/vavai/projekt/
n10http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n9http://linked.opendata.cz/ontology/domain/vavai/
n16http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n11http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
n14http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216275%3A25310%2F05%3A00002641%21RIV08-MSM-25310___/
xsdhhttp://www.w3.org/2001/XMLSchema#
n15http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n5http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F05%3A00002641%21RIV08-MSM-25310___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Epitope extraction technique is based on the specific digestion of a target protein followed by immunoaffinity isolation of a specific recognition peptide. This technique, in combination with mass spectrometry, has been efficiently used for epitope identification. The major goal of this work was to utilize newly developed enzyme and immunoaffinity magnetic reactors for the epitope extraction procedure and confirm the efficiency of this improved system for epitope screening of proteins. Alginic acid-coated magnetite microparticles with immobilized TPCK-trypsin provided high working efficiency with low non-specific adsorption, digestion time in minutes and low frequency of missed cleavages. The sensitivity and specificity of tryptic fragmentation of the beta-amyloid-peptide Abeta (1-40) as a model polypeptide was confirmed by Fourier-transform ion cyclotron resonance mass spectrometry analysis. The Sepharose reactor or immunoaffinity magnetic reactors, both with anti-amyloid-beta monoclonal antibodies, Epitope extraction technique is based on the specific digestion of a target protein followed by immunoaffinity isolation of a specific recognition peptide. This technique, in combination with mass spectrometry, has been efficiently used for epitope identification. The major goal of this work was to utilize newly developed enzyme and immunoaffinity magnetic reactors for the epitope extraction procedure and confirm the efficiency of this improved system for epitope screening of proteins. Alginic acid-coated magnetite microparticles with immobilized TPCK-trypsin provided high working efficiency with low non-specific adsorption, digestion time in minutes and low frequency of missed cleavages. The sensitivity and specificity of tryptic fragmentation of the beta-amyloid-peptide Abeta (1-40) as a model polypeptide was confirmed by Fourier-transform ion cyclotron resonance mass spectrometry analysis. The Sepharose reactor or immunoaffinity magnetic reactors, both with anti-amyloid-beta monoclonal antibodies, Extrakce epitopů - nástroj ke screeningu potencionálních peptidů vhodných pro vakcínu, za použití proteolytického magnetického reaktoru kombinovaného s FT-ICR MS.
dcterms:title
Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins Extrakce epitopů - nástroj ke screeningu potencionálních peptidů vhodných pro vakcínu, za použití proteolytického magnetického reaktoru kombinovaného s FT-ICR MS.
skos:prefLabel
Extrakce epitopů - nástroj ke screeningu potencionálních peptidů vhodných pro vakcínu, za použití proteolytického magnetického reaktoru kombinovaného s FT-ICR MS. Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins
skos:notation
RIV/00216275:25310/05:00002641!RIV08-MSM-25310___
n4:strany
489-495
n4:aktivita
n12:Z n12:P
n4:aktivity
P(GA203/05/0241), Z(MSM0021627502)
n4:cisloPeriodika
5
n4:dodaniDat
n5:2008
n4:domaciTvurceVysledku
n10:3796876 n10:9213627
n4:druhVysledku
n17:J
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
520448
n4:idVysledku
RIV/00216275:25310/05:00002641
n4:jazykVysledku
n15:eng
n4:klicovaSlova
trypsin; magnetic microspheres; Ab (1-40) peptide; epitope extraction; immunosorbent; FT-ICR-MS; vaccine lead structure
n4:klicoveSlovo
n11:epitope%20extraction n11:magnetic%20microspheres n11:vaccine%20lead%20structure n11:trypsin n11:immunosorbent n11:Ab%20%281-40%29%20peptide n11:FT-ICR-MS
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[83F874B5AB38]
n4:nazevZdroje
European Journal of Mass Spectrometry
n4:obor
n18:CB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
8
n4:projekt
n19:GA203%2F05%2F0241
n4:rokUplatneniVysledku
n5:2005
n4:svazekPeriodika
11
n4:tvurceVysledku
Ouzká, Šárka Bílková, Zuzana Stefanescu, R. Przybylski, M. Viovy, Jean-Louis Ježová, Jana Korecká, Lucie Cecal, R.
n4:zamer
n16:MSM0021627502
s:issn
1469-0667
s:numberOfPages
6
n3:organizacniJednotka
25310