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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F03%3A00006599%21RIV08-MSM-25310___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
A pilot survey of sources of contamination with arcobacters, which represent a potential risk for humans, was done in a wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.), from a retail level and domestic farming. Sanitary practices in abattoirs and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The applied method was based on a selective enrichment to isolate suspect strains, in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical for the genus Arcobacter, and a product of 686 bp, specific for A. butzleri. On the whole, a total number of 198 samples was tested, of that 33 (17%) were found to be positive for genus Arc Byla optimalizována technika PCRk detekci jednotlivých druhů arkobakterů . Ze 198 vzorků bylo pozitivních 33 (17%) pro rod Arcobacter a 22(11%) pro A. butzleri. A pilot survey of sources of contamination with arcobacters, which represent a potential risk for humans, was done in a wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.), from a retail level and domestic farming. Sanitary practices in abattoirs and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The applied method was based on a selective enrichment to isolate suspect strains, in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical for the genus Arcobacter, and a product of 686 bp, specific for A. butzleri. On the whole, a total number of 198 samples was tested, of that 33 (17%) were found to be positive for genus Arc
dcterms:title
Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from meat-Processing Plants by a Culture Technique and Detection by PCR Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from meat-Processing Plants by a Culture Technique and Detection by PCR Izolace Arcobacter butzleri a A. cryaerophilus ze vzorků masa a z masozpracujících závodů kulturními technikamia detekce pomocí PCR
skos:prefLabel
Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from meat-Processing Plants by a Culture Technique and Detection by PCR Izolace Arcobacter butzleri a A. cryaerophilus ze vzorků masa a z masozpracujících závodů kulturními technikamia detekce pomocí PCR Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from meat-Processing Plants by a Culture Technique and Detection by PCR
skos:notation
RIV/00216275:25310/03:00006599!RIV08-MSM-25310___
n3:strany
227-2327
n3:aktivita
n16:S n16:P
n3:aktivity
P(GA203/02/0023), S
n3:cisloPeriodika
2
n3:dodaniDat
n17:2008
n3:domaciTvurceVysledku
n4:7035411 n4:3777111
n3:druhVysledku
n15:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
611289
n3:idVysledku
RIV/00216275:25310/03:00006599
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Arcobacter; Meat-processing; PCR
n3:klicoveSlovo
n9:Meat-processing n9:PCR n9:Arcobacter
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[BD6AFB4DF7A1]
n3:nazevZdroje
Folia Microbiologica
n3:obor
n12:EE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n11:GA203%2F02%2F0023
n3:rokUplatneniVysledku
n17:2003
n3:svazekPeriodika
48
n3:tvurceVysledku
Pejchalová, Marcela Vytřasová, Jarmila Bínová, Šárka Harsová, Klára
s:issn
0015-5632
s:numberOfPages
7
n5:organizacniJednotka
25310