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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F03%3A00000623%21RIV08-MSM-25310___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
This work covers the possibility of using PCR method for acceleration and more accurate identification of the aflatoxinogenic fungi isolated from feed and foodstuffs. The method was optimalized on pure cultures (Aspergillus flavus CCM F-108 and Aspergillus parasiticus CCM F/550). The specificity of the optimalized PCR method was verified using various fungal strains. 50 samples of feed were examined, of which 18 were positive for the presence of the aflatoxinogenic fungi on AFPA medium. Isolated Aspergillus strains were examined using PCR method. The results obtained almost always agreed with the results gained on the AFPA medium. This method is a possible starting point for accelerating the detection of the aflatoxinogenic fungi, but it will be necessary to solve certain non-specific reactions, which are caused by a complex sample matrix. The results obtained are an initial step for further research. The PCR technique has proved itself in the detection of aflatoxinogenic fungi isolated from feed. This work covers the possibility of using PCR method for acceleration and more accurate identification of the aflatoxinogenic fungi isolated from feed and foodstuffs. The method was optimalized on pure cultures (Aspergillus flavus CCM F-108 and Aspergillus parasiticus CCM F/550). The specificity of the optimalized PCR method was verified using various fungal strains. 50 samples of feed were examined, of which 18 were positive for the presence of the aflatoxinogenic fungi on AFPA medium. Isolated Aspergillus strains were examined using PCR method. The results obtained almost always agreed with the results gained on the AFPA medium. This method is a possible starting point for accelerating the detection of the aflatoxinogenic fungi, but it will be necessary to solve certain non-specific reactions, which are caused by a complex sample matrix. The results obtained are an initial step for further research. The PCR technique has proved itself in the detection of aflatoxinogenic fungi isolated from feed. Detekce aflatoxinogenních plísní pomocí PCR metody
dcterms:title
Detection of Aflatoxinogenic Fungi in Feeds Using the PCR Method Detekce aflatoxinogenních plísní pomocí PCR metody Detection of Aflatoxinogenic Fungi in Feeds Using the PCR Method
skos:prefLabel
Detekce aflatoxinogenních plísní pomocí PCR metody Detection of Aflatoxinogenic Fungi in Feeds Using the PCR Method Detection of Aflatoxinogenic Fungi in Feeds Using the PCR Method
skos:notation
RIV/00216275:25310/03:00000623!RIV08-MSM-25310___
n3:strany
817-821
n3:aktivita
n14:Z
n3:aktivity
Z(MSM 253100002)
n3:cisloPeriodika
6
n3:dodaniDat
n16:2008
n3:domaciTvurceVysledku
n6:3777111 n6:7035411 n6:9403515 n6:7862105
n3:druhVysledku
n9:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
603251
n3:idVysledku
RIV/00216275:25310/03:00000623
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Aflatoxinogenic fungi; PCR; Aspergillus parasiticus; Aspergillus flavus
n3:klicoveSlovo
n4:Aspergillus%20flavus n4:Aflatoxinogenic%20fungi n4:PCR n4:Aspergillus%20parasiticus
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[C30BA745EF6F]
n3:nazevZdroje
Folia Microbiologica
n3:obor
n18:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:rokUplatneniVysledku
n16:2003
n3:svazekPeriodika
48
n3:tvurceVysledku
Zachová, Iveta Červenka, Libor Tavčar-Kalcher, Gabrijela Vytřasová, Jarmila Pejchalová, Marcela
n3:zamer
n8:MSM%20253100002
s:issn
0015-5632
s:numberOfPages
5
n12:organizacniJednotka
25310