This HTML5 document contains 47 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n8http://localhost/temp/predkladatel/
n13http://linked.opendata.cz/resource/domain/vavai/projekt/
n12http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n4http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14740%2F14%3A00079627%21RIV15-MSM-14740___/
n7http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
rdfshttp://www.w3.org/2000/01/rdf-schema#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n15http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n19http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n16http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n5http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00079627%21RIV15-MSM-14740___
rdf:type
n7:Vysledek skos:Concept
rdfs:seeAlso
http://pubs.acs.org/doi/abs/10.1021/am500732y
dcterms:description
Photon-upconverting nanoparticles (UCNPs) have attracted much interest as a new class of luminescent label for the background-free detection in bioanalytical applications. UCNPs and other nanoparticles are commonly coated with a silica shell to improve their dispersibility and chemical stability in aqueous buffer and to incorporate functional groups for subsequent bioconjugation steps. The process of silica coating, however, is difficult to control without suitable analytical and preparative methods. Here, we have introduced agarose gel electrophoresis for the analysis and purification of silica-coated UCNPs. The silica shell can be doped with a fluorescent dye for direct detection in the gel without influencing the structure or electrophoretic mobility of the nanoparticles. The preparation of a bare silica shell by reverse microemulsion resulted in individual nanoparticles but also distinct aggregates that could be separated and isolated from the agarose gel. Photon-upconverting nanoparticles (UCNPs) have attracted much interest as a new class of luminescent label for the background-free detection in bioanalytical applications. UCNPs and other nanoparticles are commonly coated with a silica shell to improve their dispersibility and chemical stability in aqueous buffer and to incorporate functional groups for subsequent bioconjugation steps. The process of silica coating, however, is difficult to control without suitable analytical and preparative methods. Here, we have introduced agarose gel electrophoresis for the analysis and purification of silica-coated UCNPs. The silica shell can be doped with a fluorescent dye for direct detection in the gel without influencing the structure or electrophoretic mobility of the nanoparticles. The preparation of a bare silica shell by reverse microemulsion resulted in individual nanoparticles but also distinct aggregates that could be separated and isolated from the agarose gel.
dcterms:title
Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates
skos:prefLabel
Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates
skos:notation
RIV/00216224:14740/14:00079627!RIV15-MSM-14740___
n3:aktivita
n16:P
n3:aktivity
P(ED1.1.00/02.0068), P(EE2.3.30.0009)
n3:cisloPeriodika
9
n3:dodaniDat
n5:2015
n3:domaciTvurceVysledku
n12:6669719 n12:4011236
n3:druhVysledku
n17:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
14227
n3:idVysledku
RIV/00216224:14740/14:00079627
n3:jazykVysledku
n19:eng
n3:klicovaSlova
bioconjugation; electrophoresis; nanoparticles; silica coating; upconversion
n3:klicoveSlovo
n18:bioconjugation n18:nanoparticles n18:upconversion n18:electrophoresis n18:silica%20coating
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[2F4C9CC0818A]
n3:nazevZdroje
ACS APPLIED MATERIALS & INTERFACES
n3:obor
n9:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n13:ED1.1.00%2F02.0068 n13:EE2.3.30.0009
n3:rokUplatneniVysledku
n5:2014
n3:svazekPeriodika
6
n3:tvurceVysledku
Skládal, Petr Gorris, Hans Heiner Hlaváček, Antonín Sedlmeier, Andreas
n3:wos
000336075300111
s:issn
1944-8244
s:numberOfPages
6
n15:doi
10.1021/am500732y
n8:organizacniJednotka
14740