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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00079183%21RIV15-MSM-14740___
rdf:type
n12:Vysledek skos:Concept
rdfs:seeAlso
http://download.springer.com/static/pdf/605/art%253A10.1007%252Fs00216-014-7996-0.pdf?auth66=1424693573_e49fe0b8548f527d28393c19da86150b&ext=.pdf
dcterms:description
In this paper, we present an electrochemical DNA-protein interaction assay based on a combination of protein-specific immunoprecipitation at magnetic beads (MBIP) with application of oligonucleotide (ON) probes labeled with an electroactive oxoosmium complex (Os,bipy). We show that double-stranded ONs bearing a dT(20) tail labeled with Os,bipy are specifically recognized by the tumor suppressor p53 protein according to the presence or absence of a specific binding site (p53CON) in the double-stranded segment. We demonstrate the applicability of the Os,bipy-labeled probes in titration as well as competition MBIP assays to evaluate p53 relative affinity to various sequence-specific or structurally distinct unlabeled DNA substrates upon modulation of the p53-DNA binding by monoclonal antibodies used for the immunoprecipitation. In this paper, we present an electrochemical DNA-protein interaction assay based on a combination of protein-specific immunoprecipitation at magnetic beads (MBIP) with application of oligonucleotide (ON) probes labeled with an electroactive oxoosmium complex (Os,bipy). We show that double-stranded ONs bearing a dT(20) tail labeled with Os,bipy are specifically recognized by the tumor suppressor p53 protein according to the presence or absence of a specific binding site (p53CON) in the double-stranded segment. We demonstrate the applicability of the Os,bipy-labeled probes in titration as well as competition MBIP assays to evaluate p53 relative affinity to various sequence-specific or structurally distinct unlabeled DNA substrates upon modulation of the p53-DNA binding by monoclonal antibodies used for the immunoprecipitation.
dcterms:title
Electrochemical detection of DNA binding by tumor suppressor p53 protein using osmium-labeled oligonucleotide probes and catalytic hydrogen evolution at the mercury electrode Electrochemical detection of DNA binding by tumor suppressor p53 protein using osmium-labeled oligonucleotide probes and catalytic hydrogen evolution at the mercury electrode
skos:prefLabel
Electrochemical detection of DNA binding by tumor suppressor p53 protein using osmium-labeled oligonucleotide probes and catalytic hydrogen evolution at the mercury electrode Electrochemical detection of DNA binding by tumor suppressor p53 protein using osmium-labeled oligonucleotide probes and catalytic hydrogen evolution at the mercury electrode
skos:notation
RIV/00216224:14740/14:00079183!RIV15-MSM-14740___
n3:aktivita
n7:P n7:I
n3:aktivity
I, P(GAP301/11/2076), P(IAA400040901)
n3:cisloPeriodika
24
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n14:2711435 n14:4171373
n3:druhVysledku
n4:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
14109
n3:idVysledku
RIV/00216224:14740/14:00079183
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Electrochemical analysis; Labeled probes; Osmium complex; Tumor suppressor protein p53; DNA-protein interaction; Immunoprecipitation; Competition assay; Magnetic beads; Mercury electrode
n3:klicoveSlovo
n6:Tumor%20suppressor%20protein%20p53 n6:Electrochemical%20analysis n6:Immunoprecipitation n6:Mercury%20electrode n6:Labeled%20probes n6:Osmium%20complex n6:Competition%20assay n6:Magnetic%20beads n6:DNA-protein%20interaction
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[CFD34C23BA1C]
n3:nazevZdroje
Analytical and Bioanalytical chemistry
n3:obor
n20:CG
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n18:GAP301%2F11%2F2076 n18:IAA400040901
n3:rokUplatneniVysledku
n10:2014
n3:svazekPeriodika
406
n3:tvurceVysledku
Havran, Luděk Šebest, Peter Orság, Petr Pivoňková, Hana Němcová, Kateřina Fojta, Miroslav
n3:wos
000341834500011
s:issn
1618-2642
s:numberOfPages
10
n15:doi
10.1007/s00216-014-7996-0
n13:organizacniJednotka
14740