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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00076675%21RIV15-MSM-14740___
rdf:type
n14:Vysledek skos:Concept
rdfs:seeAlso
http://rnajournal.cshlp.org/content/20/7/1112.long
dcterms:description
The hepatitis delta virus (HDV) ribozyme is a member of the class of small, self-cleaving catalytic RNAs found in a wide range of genomes from HDV to human. Both pre- and post-catalysis (precursor and product) crystal structures of the cis-acting genomic HDV ribozyme have been determined. These structures, together with extensive solution probing, have suggested that a significant conformational change accompanies catalysis. A recent crystal structure of a trans-acting precursor, obtained at low pH and by molecular replacement from the previous product conformation, conforms to the product, raising the possibility that it represents an activated conformer past the conformational change. Here, using fluorescence resonance energy transfer (FRET), we discovered that cleavage of this ribozyme at physiological pH is accompanied by a structural lengthening in magnitude comparable to previous trans-acting HDV ribozymes. The hepatitis delta virus (HDV) ribozyme is a member of the class of small, self-cleaving catalytic RNAs found in a wide range of genomes from HDV to human. Both pre- and post-catalysis (precursor and product) crystal structures of the cis-acting genomic HDV ribozyme have been determined. These structures, together with extensive solution probing, have suggested that a significant conformational change accompanies catalysis. A recent crystal structure of a trans-acting precursor, obtained at low pH and by molecular replacement from the previous product conformation, conforms to the product, raising the possibility that it represents an activated conformer past the conformational change. Here, using fluorescence resonance energy transfer (FRET), we discovered that cleavage of this ribozyme at physiological pH is accompanied by a structural lengthening in magnitude comparable to previous trans-acting HDV ribozymes.
dcterms:title
Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape
skos:prefLabel
Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape
skos:notation
RIV/00216224:14740/14:00076675!RIV15-MSM-14740___
n3:aktivita
n18:I n18:P
n3:aktivity
I, P(ED1.1.00/02.0068), P(ED2.1.00/03.0058), P(EE2.3.20.0017), P(EE2.3.20.0058), P(GAP208/12/1878)
n3:cisloPeriodika
7
n3:dodaniDat
n19:2015
n3:domaciTvurceVysledku
n20:3223779
n3:druhVysledku
n17:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
11743
n3:idVysledku
RIV/00216224:14740/14:00076675
n3:jazykVysledku
n13:eng
n3:klicovaSlova
HEPATITIS-DELTA-VIRUS; MOLECULAR-DYNAMICS SIMULATIONS; SELF-CLEAVING RIBOZYMES; ACID-BASE CATALYSIS; MULTICHANNEL REACTION-MECHANISM; LOCAL CONFORMATIONAL-CHANGES; DIVALENT METAL-IONS; GENERAL ACID; HAIRPIN RIBOZYME; GENOMIC RIBOZYME
n3:klicoveSlovo
n6:HAIRPIN%20RIBOZYME n6:ACID-BASE%20CATALYSIS n6:MOLECULAR-DYNAMICS%20SIMULATIONS n6:DIVALENT%20METAL-IONS n6:MULTICHANNEL%20REACTION-MECHANISM n6:HEPATITIS-DELTA-VIRUS n6:GENERAL%20ACID n6:GENOMIC%20RIBOZYME n6:LOCAL%20CONFORMATIONAL-CHANGES n6:SELF-CLEAVING%20RIBOZYMES
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[B934F6035378]
n3:nazevZdroje
RNA
n3:obor
n16:CF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:projekt
n9:EE2.3.20.0017 n9:GAP208%2F12%2F1878 n9:EE2.3.20.0058 n9:ED2.1.00%2F03.0058 n9:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n19:2014
n3:svazekPeriodika
20
n3:tvurceVysledku
Sripathi, Kamali N. Tay, Wendy W. Banáš, Pavel Otyepka, Michal Walter, Nils G. Šponer, Jiří
n3:wos
000338271200016
s:issn
1355-8382
s:numberOfPages
17
n10:doi
10.1261/rna.044982.114
n15:organizacniJednotka
14740