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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00075645%21RIV15-MSM-14740___
rdf:type
skos:Concept n14:Vysledek
rdfs:seeAlso
http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34
dcterms:description
Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor was applied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU x mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection of microorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved LOD was 1. Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor was applied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU x mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection of microorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved LOD was 1.
dcterms:title
Piezoelectric biosensors for detection of microorganisms Piezoelectric biosensors for detection of microorganisms
skos:prefLabel
Piezoelectric biosensors for detection of microorganisms Piezoelectric biosensors for detection of microorganisms
skos:notation
RIV/00216224:14740/14:00075645!RIV15-MSM-14740___
n4:aktivita
n12:P
n4:aktivity
P(ED1.1.00/02.0068)
n4:dodaniDat
n19:2015
n4:domaciTvurceVysledku
n5:6669719 n5:5683866 n5:1716875
n4:druhVysledku
n20:D
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
36628
n4:idVysledku
RIV/00216224:14740/14:00075645
n4:jazykVysledku
n9:eng
n4:klicovaSlova
Biosensor; Escherichia coli; Immunosensor; QCM
n4:klicoveSlovo
n6:QCM n6:Immunosensor n6:Biosensor n6:Escherichia%20coli
n4:kontrolniKodProRIV
[03DABA56B6CD]
n4:mistoKonaniAkce
Brno
n4:mistoVydani
Brno
n4:nazevZdroje
XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků
n4:obor
n22:CE
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
3
n4:projekt
n10:ED1.1.00%2F02.0068
n4:rokUplatneniVysledku
n19:2014
n4:tvurceVysledku
Farka, Zdeněk Kovář, David Skládal, Petr
n4:typAkce
n13:CST
n4:zahajeniAkce
2014-06-03+02:00
s:numberOfPages
4
n7:hasPublisher
Masarykova univerzita
n21:isbn
9788021068421
n17:organizacniJednotka
14740