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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00074131%21RIV15-MSM-14740___
rdf:type
skos:Concept n17:Vysledek
rdfs:seeAlso
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875
dcterms:description
With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated “silencing” represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein–protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach. With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated “silencing” represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein–protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach.
dcterms:title
Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies
skos:prefLabel
Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies
skos:notation
RIV/00216224:14740/14:00074131!RIV15-MSM-14740___
n4:aktivita
n19:P
n4:aktivity
P(ED1.1.00/02.0068), P(EE2.3.20.0189), P(GA13-25280S), P(GA521/09/1699), P(GAP305/11/0756)
n4:cisloPeriodika
10
n4:dodaniDat
n12:2015
n4:domaciTvurceVysledku
n6:1645102 n6:6662072 n6:9061134 n6:2299186
n4:druhVysledku
n20:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n7:predkladatel
n4:idSjednocenehoVysledku
49313
n4:idVysledku
RIV/00216224:14740/14:00074131
n4:jazykVysledku
n9:eng
n4:klicovaSlova
recombinant antibodies; immunomodulation; inhibition; protein-protein interactions; cytokinin signaling pathway
n4:klicoveSlovo
n5:recombinant%20antibodies n5:inhibition n5:immunomodulation n5:protein-protein%20interactions n5:cytokinin%20signaling%20pathway
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[0ADE81E74929]
n4:nazevZdroje
PLOS ONE : Public Library of Science
n4:obor
n14:EB
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
6
n4:projekt
n8:GA13-25280S n8:GAP305%2F11%2F0756 n8:ED1.1.00%2F02.0068 n8:GA521%2F09%2F1699 n8:EE2.3.20.0189
n4:rokUplatneniVysledku
n12:2014
n4:svazekPeriodika
9
n4:tvurceVysledku
Hrdinová, Vendula Hejátko, Jan Müller, Bruno Janda, Lubomír Conrad, Udo Zábrady, Matej
n4:wos
000343941200081
s:issn
1932-6203
s:numberOfPages
9
n11:doi
10.1371/journal.pone.0109875
n18:organizacniJednotka
14740