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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00074081%21RIV15-MSM-14740___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S002196731401036X
dcterms:description
A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1 mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3 min in a background electrolyte containing 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine and 2 mM 18-crown-6. A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1 mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3 min in a background electrolyte containing 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine and 2 mM 18-crown-6.
dcterms:title
Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
skos:prefLabel
Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
skos:notation
RIV/00216224:14740/14:00074081!RIV15-MSM-14740___
n3:aktivita
n19:P
n3:aktivity
P(ED1.1.00/02.0068), P(GA13-21919S)
n3:cisloPeriodika
September
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n4:8392137 n4:3135608 n4:7452454 n4:2428245
n3:druhVysledku
n20:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
12300
n3:idVysledku
RIV/00216224:14740/14:00074081
n3:jazykVysledku
n7:eng
n3:klicovaSlova
Capillary electrophoresis; Contactless conductivity detection; Ions; Cystic fibrosis; Principal component analysis
n3:klicoveSlovo
n12:Cystic%20fibrosis n12:Contactless%20conductivity%20detection n12:Ions n12:Principal%20component%20analysis n12:Capillary%20electrophoresis
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[13DE9C213F39]
n3:nazevZdroje
Journal of Chromatography A
n3:obor
n18:CB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n10:ED1.1.00%2F02.0068 n10:GA13-21919S
n3:rokUplatneniVysledku
n9:2014
n3:svazekPeriodika
1358
n3:tvurceVysledku
Skřičková, Jana Greguš, Michal Kubáň, Petr Pokojová, Eva Foret, František
n3:wos
000340979200035
s:issn
0021-9673
s:numberOfPages
6
n15:doi
10.1016/j.chroma.2014.06.091
n6:organizacniJednotka
14740