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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00073809%21RIV15-MSM-14740___
rdf:type
n12:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1002/jemt.22407
dcterms:description
The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope. The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope.
dcterms:title
New sensitive detection method for lectin hemagglutination using microscopy. New sensitive detection method for lectin hemagglutination using microscopy.
skos:prefLabel
New sensitive detection method for lectin hemagglutination using microscopy. New sensitive detection method for lectin hemagglutination using microscopy.
skos:notation
RIV/00216224:14740/14:00073809!RIV15-MSM-14740___
n3:aktivita
n17:P
n3:aktivity
P(ED1.1.00/02.0068), P(EE2.3.30.0009), P(GA13-25401S)
n3:cisloPeriodika
10
n3:dodaniDat
n14:2015
n3:domaciTvurceVysledku
n8:5522064 n8:3543331 n8:1636448
n3:druhVysledku
n19:J
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
32491
n3:idVysledku
RIV/00216224:14740/14:00073809
n3:jazykVysledku
n5:eng
n3:klicovaSlova
hemagglutination; protein-carbohydrate interactions; blood group antigens; lectin
n3:klicoveSlovo
n6:lectin n6:protein-carbohydrate%20interactions n6:hemagglutination n6:blood%20group%20antigens
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[384FBAADA719]
n3:nazevZdroje
Microscopy research and technique
n3:obor
n13:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n4:EE2.3.30.0009 n4:GA13-25401S n4:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n14:2014
n3:svazekPeriodika
77
n3:tvurceVysledku
Adamová, Lenka Malinovská, Lenka Wimmerová, Michaela
n3:wos
000342207900012
s:issn
1059-910X
s:numberOfPages
9
n7:doi
10.1002/jemt.22407
n9:organizacniJednotka
14740