This HTML5 document contains 59 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n19http://localhost/temp/predkladatel/
n11http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n7http://linked.opendata.cz/resource/domain/vavai/projekt/
n5http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
rdfshttp://www.w3.org/2000/01/rdf-schema#
skoshttp://www.w3.org/2004/02/skos/core#
n16http://bibframe.org/vocab/
n12http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14740%2F14%3A00073698%21RIV15-MSM-14740___/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n6http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n20http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n17http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n9http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00073698%21RIV15-MSM-14740___
rdf:type
skos:Concept n5:Vysledek
rdfs:seeAlso
http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm
dcterms:description
The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome. The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome.
dcterms:title
In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception
skos:prefLabel
In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception
skos:notation
RIV/00216224:14740/14:00073698!RIV15-MSM-14740___
n4:aktivita
n17:I n17:S n17:P
n4:aktivity
I, P(ED1.1.00/02.0068), P(GAP501/11/1462), P(GPP501/11/P321), P(LD13049), S
n4:cisloPeriodika
April
n4:dodaniDat
n9:2015
n4:domaciTvurceVysledku
n11:6563902 n11:4568567
n4:druhVysledku
n14:J
n4:duvernostUdaju
n20:S
n4:entitaPredkladatele
n12:predkladatel
n4:idSjednocenehoVysledku
21048
n4:idVysledku
RIV/00216224:14740/14:00073698
n4:jazykVysledku
n15:eng
n4:klicovaSlova
GPI-ANCHORED PROTEIN; PLANT SECRETOME; ARABIDOPSIS; GROWTH; GUIDANCE; GERMINATION; ATTRACTANT; COMPONENTS; MAIZE; CELL
n4:klicoveSlovo
n6:COMPONENTS n6:MAIZE n6:CELL n6:PLANT%20SECRETOME n6:GERMINATION n6:GPI-ANCHORED%20PROTEIN n6:GROWTH n6:GUIDANCE n6:ATTRACTANT n6:ARABIDOPSIS
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[AE6B3A0FB0BA]
n4:nazevZdroje
BIOCHEMICAL SOCIETY TRANSACTIONS
n4:obor
n18:EF
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
7
n4:projekt
n7:LD13049 n7:GAP501%2F11%2F1462 n7:ED1.1.00%2F02.0068 n7:GPP501%2F11%2FP321
n4:rokUplatneniVysledku
n9:2014
n4:svazekPeriodika
42
n4:tvurceVysledku
Honys, David Fecíková, Jana Zdráhal, Zbyněk Čapková, Věra Hafidh, Said Potěšil, David Fíla, Jan
n4:wos
000333658900028
s:issn
0300-5127
s:numberOfPages
7
n16:doi
10.1042/BST20130204
n19:organizacniJednotka
14740