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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00073613%21RIV15-MSM-14740___
rdf:type
n4:Vysledek skos:Concept
rdfs:seeAlso
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186968/
dcterms:description
The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of the released transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, but the mechanism underlying this coupling remains enigmatic. Here we provide structural and functional evidence demonstrating that the same domain of Nrd1p interacts with RNA polymerase II and Trf4p in a mutually exclusive manner, thus defining two alternative forms of the NNS complex, one involved in termination and the other in degradation. We show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro. The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of the released transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, but the mechanism underlying this coupling remains enigmatic. Here we provide structural and functional evidence demonstrating that the same domain of Nrd1p interacts with RNA polymerase II and Trf4p in a mutually exclusive manner, thus defining two alternative forms of the NNS complex, one involved in termination and the other in degradation. We show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro.
dcterms:title
Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation
skos:prefLabel
Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation
skos:notation
RIV/00216224:14740/14:00073613!RIV15-MSM-14740___
n6:aktivita
n15:P
n6:aktivity
P(ED1.1.00/02.0068), P(EE2.3.20.0042), P(GA13-18344S), P(GBP305/12/G034)
n6:cisloPeriodika
3
n6:dodaniDat
n12:2015
n6:domaciTvurceVysledku
n14:9123377 n14:6439608 Kabzinski, Tomasz n14:5234360 n14:7878788
n6:druhVysledku
n17:J
n6:duvernostUdaju
n9:S
n6:entitaPredkladatele
n16:predkladatel
n6:idSjednocenehoVysledku
30269
n6:idVysledku
RIV/00216224:14740/14:00073613
n6:jazykVysledku
n18:eng
n6:klicovaSlova
CRYPTIC UNSTABLE TRANSCRIPTS; POLYMERASE-II TRANSCRIPTS; BINDING PROTEINS NRD1; QUALITY-CONTROL; SACCHAROMYCES-CEREVISIAE; PERVASIVE TRANSCRIPTION; BIDIRECTIONAL PROMOTERS; POLY(A) POLYMERASE; NUCLEAR EXOSOME; TRAMP COMPLEX
n6:klicoveSlovo
n10:QUALITY-CONTROL n10:TRAMP%20COMPLEX n10:SACCHAROMYCES-CEREVISIAE n10:POLY%28A%29%20POLYMERASE n10:NUCLEAR%20EXOSOME n10:CRYPTIC%20UNSTABLE%20TRANSCRIPTS n10:PERVASIVE%20TRANSCRIPTION n10:BIDIRECTIONAL%20PROMOTERS n10:BINDING%20PROTEINS%20NRD1 n10:POLYMERASE-II%20TRANSCRIPTS
n6:kodStatuVydavatele
US - Spojené státy americké
n6:kontrolniKodProRIV
[56AFFEF6C33C]
n6:nazevZdroje
Molecular Cell
n6:obor
n11:BO
n6:pocetDomacichTvurcuVysledku
5
n6:pocetTvurcuVysledku
11
n6:projekt
n7:ED1.1.00%2F02.0068 n7:GA13-18344S n7:GBP305%2F12%2FG034 n7:EE2.3.20.0042
n6:rokUplatneniVysledku
n12:2014
n6:svazekPeriodika
55
n6:tvurceVysledku
Lacroute, Francoise Lidschreiber, Michael Cramer, Patrick Tudek, Agnieszka Fořtová, Andrea Vaňáčová, Štěpánka Libri, Domenico Kubíček, Karel Kabzinski, Tomasz Štefl, Richard Fuerte, Odil Porrua
n6:wos
000340646600012
s:issn
1097-2765
s:numberOfPages
15
n3:doi
10.1016/j.molcel.2014.05.031
n20:organizacniJednotka
14740