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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F14%3A00073589%21RIV15-MSM-14740___
rdf:type
n12:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1016/j.electacta.2013.09.147
dcterms:description
A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications. A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.
dcterms:title
Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode
skos:prefLabel
Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode
skos:notation
RIV/00216224:14740/14:00073589!RIV15-MSM-14740___
n3:aktivita
n13:Z n13:P n13:I
n3:aktivity
I, P(EE2.3.30.0019), P(GBP206/12/G151), Z(AV0Z50040702)
n3:cisloPeriodika
SI
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n10:4171373
n3:druhVysledku
n16:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
14097
n3:idVysledku
RIV/00216224:14740/14:00073589
n3:jazykVysledku
n19:eng
n3:klicovaSlova
DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry
n3:klicoveSlovo
n7:Silver%20solid%20amalgam%20electrode n7:2_4-Dinitrophenylhydrazine n7:Voltammetry n7:DNA n7:Redox%20labeling
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[767E3DF4C849]
n3:nazevZdroje
Electrochimica Acta
n3:obor
n6:CG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:projekt
n15:EE2.3.30.0019 n15:GBP206%2F12%2FG151
n3:rokUplatneniVysledku
n9:2014
n3:svazekPeriodika
126
n3:tvurceVysledku
Hocek, Michal Raindlova, Veronika Daňhel, Aleš Pivoňková, Hana Fojta, Miroslav Havran, Luděk
n3:wos
000336340900016
n3:zamer
n17:AV0Z50040702
s:issn
0013-4686
s:numberOfPages
10
n21:doi
10.1016/j.electacta.2013.09.147
n11:organizacniJednotka
14740