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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F13%3A00069571%21RIV14-MSM-14740___
rdf:type
skos:Concept n14:Vysledek
rdfs:seeAlso
http://onlinelibrary.wiley.com/doi/10.1002/ijch.201200074/abstract
dcterms:description
Frequently used nucleosome mapping techniques, both experimental and computational ones, pursue a rather simple task to determine whether a given sequence segment is likely to belong to a nucleosome, thus measuring the nucleosome occupancy along the sequence. A more ambitious task is to determine the position with high resolution, so that not only the approximate translational position of the nucleosome on DNA would be known, but also the rotational setting of the DNA. The rotational setting is important to know since the binding of various transcription factors to the nucleosome DNA crucially depends on the accessibility of the respective recognition sequences. The only experimental technique that provides the highest possible accuracy of the positioning is crystallization of the nucleosomes reconstituted on specific sequences, with subsequent solving of their structures from x-ray diffraction data. Frequently used nucleosome mapping techniques, both experimental and computational ones, pursue a rather simple task to determine whether a given sequence segment is likely to belong to a nucleosome, thus measuring the nucleosome occupancy along the sequence. A more ambitious task is to determine the position with high resolution, so that not only the approximate translational position of the nucleosome on DNA would be known, but also the rotational setting of the DNA. The rotational setting is important to know since the binding of various transcription factors to the nucleosome DNA crucially depends on the accessibility of the respective recognition sequences. The only experimental technique that provides the highest possible accuracy of the positioning is crystallization of the nucleosomes reconstituted on specific sequences, with subsequent solving of their structures from x-ray diffraction data.
dcterms:title
Single-Base Resolution Sequence-Directed Nucleosome Mapping Single-Base Resolution Sequence-Directed Nucleosome Mapping
skos:prefLabel
Single-Base Resolution Sequence-Directed Nucleosome Mapping Single-Base Resolution Sequence-Directed Nucleosome Mapping
skos:notation
RIV/00216224:14740/13:00069571!RIV14-MSM-14740___
n14:predkladatel
n15:orjk%3A14740
n4:aktivita
n18:Z
n4:aktivity
Z(MSM0021622415)
n4:cisloPeriodika
3-4
n4:dodaniDat
n7:2014
n4:domaciTvurceVysledku
Trifonov, Eduard Nikolajevič n12:8855404
n4:druhVysledku
n13:J
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n6:predkladatel
n4:idSjednocenehoVysledku
105336
n4:idVysledku
RIV/00216224:14740/13:00069571
n4:jazykVysledku
n10:eng
n4:klicovaSlova
DNA; ucleobases; nucleosome positioning; regulation of transcription; TATA box
n4:klicoveSlovo
n5:regulation%20of%20transcription n5:DNA n5:TATA%20box n5:nucleosome%20positioning n5:ucleobases
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[FA6911294B2C]
n4:nazevZdroje
Israel Journal of Chemistry
n4:obor
n21:EB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:rokUplatneniVysledku
n7:2013
n4:svazekPeriodika
53
n4:tvurceVysledku
Trifonov, Eduard Nikolajevič Hapala, Jan
n4:wos
000317859800003
n4:zamer
n20:MSM0021622415
s:issn
0021-2148
s:numberOfPages
3
n11:doi
10.1002/ijch.201200074
n17:organizacniJednotka
14740