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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F13%3A00066469%21RIV14-MSM-14740___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Metals play a crucial role in physiology and pathology of biological systems. It has been estimated that the metalloproteins encompass about one third of all proteins; they are involved in the regulation of protein expression, metal transportation, homeostasis or detoxification process. A novel method for comprehensive multidimensional analysis of metalloproteins is presented here. This approach is based on an off-line coupling of a single micro-column separation run to both substrate-assisted laser desorption (SALD) inductively coupled plasma (ICP) mass spectrometry (MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI) MS. The effluent fractions are collected on a custom-designed Au-coated polyethylene terephthalate glycol (PETG) sample target that is compatible to both MS methods. Metals play a crucial role in physiology and pathology of biological systems. It has been estimated that the metalloproteins encompass about one third of all proteins; they are involved in the regulation of protein expression, metal transportation, homeostasis or detoxification process. A novel method for comprehensive multidimensional analysis of metalloproteins is presented here. This approach is based on an off-line coupling of a single micro-column separation run to both substrate-assisted laser desorption (SALD) inductively coupled plasma (ICP) mass spectrometry (MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI) MS. The effluent fractions are collected on a custom-designed Au-coated polyethylene terephthalate glycol (PETG) sample target that is compatible to both MS methods.
dcterms:title
Comprehensive metalloprotein analysis from a single separation record Comprehensive metalloprotein analysis from a single separation record
skos:prefLabel
Comprehensive metalloprotein analysis from a single separation record Comprehensive metalloprotein analysis from a single separation record
skos:notation
RIV/00216224:14740/13:00066469!RIV14-MSM-14740___
n15:predkladatel
n16:orjk%3A14740
n3:aktivita
n11:S n11:P
n3:aktivity
P(ED1.1.00/02.0068), P(GAP206/12/0538), S
n3:dodaniDat
n12:2014
n3:domaciTvurceVysledku
n5:9236333 n5:7026390 n5:2647788 n5:6610528
n3:druhVysledku
n10:O
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
66535
n3:idVysledku
RIV/00216224:14740/13:00066469
n3:jazykVysledku
n13:eng
n3:klicovaSlova
metalloproteins; separation; mass spectrometry; hyphenated techniques
n3:klicoveSlovo
n4:separation n4:metalloproteins n4:mass%20spectrometry n4:hyphenated%20techniques
n3:kontrolniKodProRIV
[AD9E20D59F46]
n3:obor
n7:CB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n18:GAP206%2F12%2F0538 n18:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n12:2013
n3:tvurceVysledku
Foltynová, Pavla Kanický, Viktor Tomalová, Iva Preisler, Jan
n17:organizacniJednotka
14740