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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F12%3A00058010%21RIV13-GA0-14740___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
Gold nanoparticles (GNPs) represent a very attractive material for the construction of sensors and biosensors with immobilized aptamer on the surface, due to their biocompatibility. The most extensively studied aptamer is the thrombin binding aptamer (TBA) with sequence GGT TGG TGT GGT TGG, specifically binding to thrombin, and thus inhibits the activity of thrombin in the cascade of reactions resulting in the hemocoagulation. The aim of our contribution is spectroscopic characterization of aptasensor system and verify its specificity to thrombin in buffered and non – buffered aqueous solutions with adjusted ionic strength. For our experiment the GNPs (5, 10 and 20nm) stabilized in citrate buffer were used. The absorption spectra were monitored not only in the dependence on particles size, but also on the components of used solutions. For the detection of aggregation and behavior of unmodified and modified GNPs the distribution of GNPs and their zeta potential were determined. Gold nanoparticles (GNPs) represent a very attractive material for the construction of sensors and biosensors with immobilized aptamer on the surface, due to their biocompatibility. The most extensively studied aptamer is the thrombin binding aptamer (TBA) with sequence GGT TGG TGT GGT TGG, specifically binding to thrombin, and thus inhibits the activity of thrombin in the cascade of reactions resulting in the hemocoagulation. The aim of our contribution is spectroscopic characterization of aptasensor system and verify its specificity to thrombin in buffered and non – buffered aqueous solutions with adjusted ionic strength. For our experiment the GNPs (5, 10 and 20nm) stabilized in citrate buffer were used. The absorption spectra were monitored not only in the dependence on particles size, but also on the components of used solutions. For the detection of aggregation and behavior of unmodified and modified GNPs the distribution of GNPs and their zeta potential were determined.
dcterms:title
Spektroskopická charakterizace aptasenzoru specifického pro thrombin Spektroskopická charakterizace aptasenzoru specifického pro thrombin
skos:prefLabel
Spektroskopická charakterizace aptasenzoru specifického pro thrombin Spektroskopická charakterizace aptasenzoru specifického pro thrombin
skos:notation
RIV/00216224:14740/12:00058010!RIV13-GA0-14740___
n7:predkladatel
n9:orjk%3A14740
n3:aktivita
n20:P
n3:aktivity
P(GD106/09/H035)
n3:dodaniDat
n8:2013
n3:domaciTvurceVysledku
n12:6359922
n3:druhVysledku
n22:D
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
170404
n3:idVysledku
RIV/00216224:14740/12:00058010
n3:jazykVysledku
n21:eng
n3:klicovaSlova
Gold nanoparticles; GNPs; TBA; thrombin; aptamer; zeta potential
n3:klicoveSlovo
n5:TBA n5:GNPs n5:Gold%20nanoparticles n5:thrombin n5:aptamer n5:zeta%20potential
n3:kontrolniKodProRIV
[AB8E120B7413]
n3:mistoKonaniAkce
Brno
n3:mistoVydani
Brno
n3:nazevZdroje
Víceúrovňový design pokrokových materiálů, sborník doktorské konference 2012
n3:obor
n15:CF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:projekt
n17:GD106%2F09%2FH035
n3:rokUplatneniVysledku
n8:2012
n3:tvurceVysledku
Pilařová, Iveta Polsterová, Svatava Trnková, Libuše
n3:typAkce
n11:CST
n3:zahajeniAkce
2012-01-01+01:00
s:numberOfPages
8
n13:hasPublisher
Ústav fyziky materiálů AV ČR
n14:isbn
9788087434062
n18:organizacniJednotka
14740