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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F12%3A00057434%21RIV13-GA0-14740___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Gold nanoparticles (GNPs) are the object of interest in the field of nanotechnology due to their unique electronic, structural, optical, magnetic, thermal, and catalytic properties. They are extensively applied in the construction of electrochemical sensors and biosensors. The aim of this contribution is to characterize GNPs of both sizes (diameters of 5nm and 10 nm) in buffered and non-buffered aqueous solutions with the change of ionic strength by spectroscopic methods (UV/ Vis, photon correlation spectroscopy), and to investigate the GNP–aptamer–thrombin system as an application of GNPs in a sensitive aptasensor. For the immobilization the thrombin-binding aptamer (TBA) with the sequence GGT TGG TGT GGT TGG on the GNP surface was modified by a sulfhydryl group. The study showed that the spectral characterization of GNPs depends strongly not only on the aptamer–thrombin conjugate but also on the GNP size and solution composition (buffer, ions and their concentrations). Gold nanoparticles (GNPs) are the object of interest in the field of nanotechnology due to their unique electronic, structural, optical, magnetic, thermal, and catalytic properties. They are extensively applied in the construction of electrochemical sensors and biosensors. The aim of this contribution is to characterize GNPs of both sizes (diameters of 5nm and 10 nm) in buffered and non-buffered aqueous solutions with the change of ionic strength by spectroscopic methods (UV/ Vis, photon correlation spectroscopy), and to investigate the GNP–aptamer–thrombin system as an application of GNPs in a sensitive aptasensor. For the immobilization the thrombin-binding aptamer (TBA) with the sequence GGT TGG TGT GGT TGG on the GNP surface was modified by a sulfhydryl group. The study showed that the spectral characterization of GNPs depends strongly not only on the aptamer–thrombin conjugate but also on the GNP size and solution composition (buffer, ions and their concentrations).
dcterms:title
SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES
skos:prefLabel
SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES
skos:notation
RIV/00216224:14740/12:00057434!RIV13-GA0-14740___
n12:predkladatel
n13:orjk%3A14740
n3:aktivita
n7:P
n3:aktivity
P(ED1.1.00/02.0068), P(GD106/09/H035)
n3:dodaniDat
n10:2013
n3:domaciTvurceVysledku
n18:6359922 n18:3790185
n3:druhVysledku
n11:D
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
170370
n3:idVysledku
RIV/00216224:14740/12:00057434
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Gold nanoparticles; GNPs; UV-Vis spectroscopy; PCS; thrombin; aptamer
n3:klicoveSlovo
n5:thrombin n5:Gold%20nanoparticles n5:aptamer n5:GNPs n5:PCS n5:UV-Vis%20spectroscopy
n3:kontrolniKodProRIV
[E88057F9BF5F]
n3:mistoKonaniAkce
Brno
n3:mistoVydani
Brno
n3:nazevZdroje
XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků.
n3:obor
n16:CF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n4:GD106%2F09%2FH035 n4:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n10:2012
n3:tvurceVysledku
Castillo, Gabriela Trnková, Libuše Pilařová, Iveta
n3:typAkce
n14:EUR
n3:zahajeniAkce
2012-05-30+02:00
s:numberOfPages
4
n20:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně
n19:isbn
9788073756185
n21:organizacniJednotka
14740