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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F11%3A00050180%21RIV12-GA0-14740___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
rotein-carbohydrate interactions are usually characterized by a low affinity for monovalent ligands that is balanced by multivalency resulting in high avidity for ligands with several potential epitops. However, recent characterization of lectins involved in pathogenesis has demonstrated their much higher affinity even towards monosaccharide than that observed for plant or animal lectins [1]. This feature allows to use such lectins as a more/less specific tool, for example, for evaluation of protein glycosylation or isolation of glyco-conjugates. Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. In our work several methodical directions have been applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA. rotein-carbohydrate interactions are usually characterized by a low affinity for monovalent ligands that is balanced by multivalency resulting in high avidity for ligands with several potential epitops. However, recent characterization of lectins involved in pathogenesis has demonstrated their much higher affinity even towards monosaccharide than that observed for plant or animal lectins [1]. This feature allows to use such lectins as a more/less specific tool, for example, for evaluation of protein glycosylation or isolation of glyco-conjugates. Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. In our work several methodical directions have been applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA.
dcterms:title
Protein engineering of lectins - in silico and in vitro approaches Protein engineering of lectins - in silico and in vitro approaches
skos:prefLabel
Protein engineering of lectins - in silico and in vitro approaches Protein engineering of lectins - in silico and in vitro approaches
skos:notation
RIV/00216224:14740/11:00050180!RIV12-GA0-14740___
n10:predkladatel
n16:orjk%3A14740
n3:aktivita
n15:P n15:Z
n3:aktivity
P(GAP207/10/0321), P(GPP207/11/P185), Z(MSM0021622413)
n3:dodaniDat
n13:2012
n3:domaciTvurceVysledku
n8:7678037 n8:6666477 n8:5616697 n8:5522064 n8:4347374
n3:druhVysledku
n14:O
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
224813
n3:idVysledku
RIV/00216224:14740/11:00050180
n3:jazykVysledku
n11:eng
n3:klicovaSlova
lectin; error prone PCR; random mutagenesis
n3:klicoveSlovo
n9:random%20mutagenesis n9:error%20prone%20PCR n9:lectin
n3:kontrolniKodProRIV
[B68CC778CCD3]
n3:obor
n18:CE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n5:GPP207%2F11%2FP185 n5:GAP207%2F10%2F0321
n3:rokUplatneniVysledku
n13:2011
n3:tvurceVysledku
Wimmerová, Michaela Adam, Jan Mrázková, Jana Koča, Jaroslav Pokorná, Martina
n3:zamer
n12:MSM0021622413
n4:organizacniJednotka
14740