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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F11%3A00050138%21RIV12-GA0-14740___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Directed evolution is widely used in protein engineering for producing proteins with altered or improved properties. This powerful tool includes several main approaches. The first is a random mutagenesis, which is used for creating random mutations anywhere in the gene of interest. The second is a targeted random mutagenesis, which is utilized for preparing mutants with random base substitutions in selected hotspots. In addition, a site directed mutagenesis is based on rational design and it serves for creating specific amino acid substitutions in selected hotspots. In our work two technical approaches - the targeted-random mutagenesis via mixture of oligonucleotide primers and the site directed mutagenesis via two primers carrying specific substitution – are employed. Lectins are proteins produced by different organisms. They are able to bind various sugars specifically. Directed evolution is widely used in protein engineering for producing proteins with altered or improved properties. This powerful tool includes several main approaches. The first is a random mutagenesis, which is used for creating random mutations anywhere in the gene of interest. The second is a targeted random mutagenesis, which is utilized for preparing mutants with random base substitutions in selected hotspots. In addition, a site directed mutagenesis is based on rational design and it serves for creating specific amino acid substitutions in selected hotspots. In our work two technical approaches - the targeted-random mutagenesis via mixture of oligonucleotide primers and the site directed mutagenesis via two primers carrying specific substitution – are employed. Lectins are proteins produced by different organisms. They are able to bind various sugars specifically.
dcterms:title
Directed evolution of lectins from pathogenic bacteria Directed evolution of lectins from pathogenic bacteria
skos:prefLabel
Directed evolution of lectins from pathogenic bacteria Directed evolution of lectins from pathogenic bacteria
skos:notation
RIV/00216224:14740/11:00050138!RIV12-GA0-14740___
n16:predkladatel
n17:orjk%3A14740
n3:aktivita
n13:S n13:P n13:Z
n3:aktivity
P(GA303/09/1168), P(GD301/09/H004), P(LC06030), S, Z(MSM0021622413)
n3:dodaniDat
n5:2012
n3:domaciTvurceVysledku
n7:5522064 n7:5616697 n7:7105886 n7:6666477
n3:druhVysledku
n12:O
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
194761
n3:idVysledku
RIV/00216224:14740/11:00050138
n3:jazykVysledku
n8:eng
n3:klicovaSlova
directed evolution; random mutagenesis in vitro; targeted-random mutagenesis; site-directed mutagenersis; lectins; PA-IIL; Pseudomonas aeruginosa; RS-IIL; Ralstonia solanacearum; BC2L-A; Burkholderia cenocepacia
n3:klicoveSlovo
n4:BC2L-A n4:random%20mutagenesis%20in%20vitro n4:Ralstonia%20solanacearum n4:targeted-random%20mutagenesis n4:lectins n4:RS-IIL n4:PA-IIL n4:Pseudomonas%20aeruginosa n4:directed%20evolution n4:site-directed%20mutagenersis n4:Burkholderia%20cenocepacia
n3:kontrolniKodProRIV
[5EF2CA98992F]
n3:obor
n19:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n9:GD301%2F09%2FH004 n9:GA303%2F09%2F1168 n9:LC06030
n3:rokUplatneniVysledku
n5:2011
n3:tvurceVysledku
Pokorná, Martina Safy, Marek Wimmerová, Michaela Mrázková, Jana
n3:zamer
n6:MSM0021622413
n15:organizacniJednotka
14740