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Statements

Subject Item
n2:RIV%2F00216224%3A14740%2F11%3A00049774%21RIV12-GA0-14740___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
The structure of N-terminal domain was determined by NMR. It consists mainly of three alpha-helixes and one short beta-sheet, yet the N-terminal part remains unstructured. The cause of this flexibility is probably the His6-Tag attached at the N-terminus.The N-terminal domain of delta subunit was conquested to high-throughput screening (sitting drop), where several crystallization conditions were found.Higher quality crystals were obtained after 7-9 days with an average size 150 x 100 micrometers.Diffraction data were collected at ESRF Grenoble, ID-23. The resolution of the structure was 3.5 Angstrom. The data were processed by MOSFLM and the determination of the structure was done by molecular replacement with NMR structure used as a model. The structure of N-terminal domain was determined by NMR. It consists mainly of three alpha-helixes and one short beta-sheet, yet the N-terminal part remains unstructured. The cause of this flexibility is probably the His6-Tag attached at the N-terminus.The N-terminal domain of delta subunit was conquested to high-throughput screening (sitting drop), where several crystallization conditions were found.Higher quality crystals were obtained after 7-9 days with an average size 150 x 100 micrometers.Diffraction data were collected at ESRF Grenoble, ID-23. The resolution of the structure was 3.5 Angstrom. The data were processed by MOSFLM and the determination of the structure was done by molecular replacement with NMR structure used as a model.
dcterms:title
Crystallization and structure determination of delta-subunit of RNApolymerase Crystallization and structure determination of delta-subunit of RNApolymerase
skos:prefLabel
Crystallization and structure determination of delta-subunit of RNApolymerase Crystallization and structure determination of delta-subunit of RNApolymerase
skos:notation
RIV/00216224:14740/11:00049774!RIV12-GA0-14740___
n15:predkladatel
n16:orjk%3A14740
n4:aktivita
n8:Z n8:P
n4:aktivity
P(GA204/09/0583), P(GD301/09/H004), Z(MSM0021622413)
n4:dodaniDat
n5:2012
n4:domaciTvurceVysledku
n6:7064322 n6:5522064 n6:4257707 n6:3489418
n4:druhVysledku
n17:O
n4:duvernostUdaju
n7:S
n4:entitaPredkladatele
n19:predkladatel
n4:idSjednocenehoVysledku
192371
n4:idVysledku
RIV/00216224:14740/11:00049774
n4:jazykVysledku
n14:eng
n4:klicovaSlova
delta-subunit RNA polymerase crystallization structure
n4:klicoveSlovo
n11:delta-subunit%20RNA%20polymerase%20crystallization%20structure
n4:kontrolniKodProRIV
[E187533C253D]
n4:obor
n18:CE
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
5
n4:projekt
n13:GD301%2F09%2FH004 n13:GA204%2F09%2F0583
n4:rokUplatneniVysledku
n5:2011
n4:tvurceVysledku
Wimmerová, Michaela Šanderová, Hana Demo, Gabriel Žídek, Lukáš Papoušková, Veronika
n4:zamer
n9:MSM0021622413
n12:organizacniJednotka
14740