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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F12%3A00057522%21RIV13-GA0-14330___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines.
dcterms:title
Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I
skos:prefLabel
Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I
skos:notation
RIV/00216224:14330/12:00057522!RIV13-GA0-14330___
n12:predkladatel
n13:orjk%3A14330
n3:aktivita
n19:P
n3:aktivity
P(GBP302/12/G157)
n3:cisloPeriodika
3
n3:dodaniDat
n11:2013
n3:domaciTvurceVysledku
n6:3283038 n6:3021475 n6:7460651 n6:1723162 n6:9465146
n3:druhVysledku
n17:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
144834
n3:idVysledku
RIV/00216224:14330/12:00057522
n3:jazykVysledku
n14:eng
n3:klicovaSlova
superoxide; fluorescence microscopy; image analysis; mitochondria; telomerase
n3:klicoveSlovo
n5:fluorescence%20microscopy n5:image%20analysis n5:telomerase n5:mitochondria n5:superoxide
n3:kodStatuVydavatele
AR - Argentinská republika
n3:kontrolniKodProRIV
[9D46A46D507B]
n3:nazevZdroje
BIOCELL
n3:obor
n10:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n15:GBP302%2F12%2FG157
n3:rokUplatneniVysledku
n11:2012
n3:svazekPeriodika
36
n3:tvurceVysledku
Vařecha, Miroslav Matula, Pavel Páclová, Daniela Procházková, Jiřina Kozubek, Michal Cmarko, Dušan
s:issn
0327-9545
s:numberOfPages
6
n16:organizacniJednotka
14330