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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F10%3A00067250%21RIV14-MSM-14330___
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Isolation of granulocyte cells from blood is necessary for accurate studying of changes in their expression. After gradient centrifugation, we obtain relatively pure granulocyte populations with different ratios of neutrophils and eosinophils. Unfortunately, in many studies in this field the expression results are not set according to the real variability of the granulocyte population. In many cases, the granulocyte population is marked simply as neutrophils and the residual population of eosinophils is not considered. Based on our recent study where we tracked the general transcription factor RNA polymerase II (RNAP II), we hypothesized that eosinophils are more transcriptionally active cells than neutrophils. We decided to test our hypothesis on isolated cells because its implications could change our view on many past expression analyses performed on granulocytes. In our experiments, we isolated neutrophils and eosinophils and measured their total RNA production. Isolation of granulocyte cells from blood is necessary for accurate studying of changes in their expression. After gradient centrifugation, we obtain relatively pure granulocyte populations with different ratios of neutrophils and eosinophils. Unfortunately, in many studies in this field the expression results are not set according to the real variability of the granulocyte population. In many cases, the granulocyte population is marked simply as neutrophils and the residual population of eosinophils is not considered. Based on our recent study where we tracked the general transcription factor RNA polymerase II (RNAP II), we hypothesized that eosinophils are more transcriptionally active cells than neutrophils. We decided to test our hypothesis on isolated cells because its implications could change our view on many past expression analyses performed on granulocytes. In our experiments, we isolated neutrophils and eosinophils and measured their total RNA production.
dcterms:title
Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils? Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?
skos:prefLabel
Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils? Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?
skos:notation
RIV/00216224:14330/10:00067250!RIV14-MSM-14330___
n3:aktivita
n8:Z n8:P
n3:aktivity
P(2B06052), Z(MSM0021622419), Z(MSM0021622430)
n3:cisloPeriodika
6
n3:dodaniDat
n17:2014
n3:domaciTvurceVysledku
n14:2346257 n14:4748891 n14:8836086
n3:druhVysledku
n19:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
265016
n3:idVysledku
RIV/00216224:14330/10:00067250
n3:jazykVysledku
n15:eng
n3:klicovaSlova
total RNA; gradient centrifugation; cell isolation; magnetic separation; gene expression; expression activity; neutrophils; eosinophils; granulocytes
n3:klicoveSlovo
n6:cell%20isolation n6:neutrophils n6:magnetic%20separation n6:gradient%20centrifugation n6:total%20RNA n6:eosinophils n6:granulocytes n6:expression%20activity n6:gene%20expression
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[5234044AFEC6]
n3:nazevZdroje
FOLIA BIOLOGICA
n3:obor
n4:EC
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n18:2B06052
n3:rokUplatneniVysledku
n17:2010
n3:svazekPeriodika
56
n3:tvurceVysledku
Ručka, Zdeněk Krontorád Koutná, Irena Stejskal, Stanislav
n3:wos
000286039500003
n3:zamer
n9:MSM0021622419 n9:MSM0021622430
s:issn
0015-5500
s:numberOfPages
4
n16:organizacniJednotka
14330