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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F10%3A00045338%21RIV11-MSM-14330___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
Apart from this function in cell death, AIF also plays largely unknown role in cell life. We used AIF gene silencing using shRNA vectors followed by measurements by flow cytometry and confocal fluorescent microscopy to detect the superoxide production during oxidative phosphorylation in mitochondria. We managed to knock-down the expression of gene AIF by transient or stable transfection in various human cell lines. Interestingly, mitochondrial superoxide production in various cell lines reacted to gene silencing of AIF differently. Cell line U-2 OS transiently and also stably transfected with vector shRNA was found to produce less superoxide in comparison to control U-2 OS cells. Interestingly, HeLa cell line shows opposite results with significantly increased mitochondrial superoxide production in silenced HeLa cells. We are also in process of examination of other healthy and cancer cell. Apart from this function in cell death, AIF also plays largely unknown role in cell life. We used AIF gene silencing using shRNA vectors followed by measurements by flow cytometry and confocal fluorescent microscopy to detect the superoxide production during oxidative phosphorylation in mitochondria. We managed to knock-down the expression of gene AIF by transient or stable transfection in various human cell lines. Interestingly, mitochondrial superoxide production in various cell lines reacted to gene silencing of AIF differently. Cell line U-2 OS transiently and also stably transfected with vector shRNA was found to produce less superoxide in comparison to control U-2 OS cells. Interestingly, HeLa cell line shows opposite results with significantly increased mitochondrial superoxide production in silenced HeLa cells. We are also in process of examination of other healthy and cancer cell.
dcterms:title
Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell line Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell line
skos:prefLabel
Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell line Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell line
skos:notation
RIV/00216224:14330/10:00045338!RIV11-MSM-14330___
n4:aktivita
n16:P n16:Z
n4:aktivity
P(2B06052), P(LC535), Z(MSM0021622419)
n4:dodaniDat
n9:2011
n4:domaciTvurceVysledku
n8:3021475 n8:9465146 n8:1723162 n8:3283038
n4:druhVysledku
n14:O
n4:duvernostUdaju
n5:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
246016
n4:idVysledku
RIV/00216224:14330/10:00045338
n4:jazykVysledku
n10:eng
n4:klicovaSlova
AIF; shRNA; gene silencing; image analysis; superoxide; mitochondria; oxidative phosphorylation
n4:klicoveSlovo
n6:shRNA n6:gene%20silencing n6:AIF n6:mitochondria n6:image%20analysis n6:oxidative%20phosphorylation n6:superoxide
n4:kontrolniKodProRIV
[A2B209594426]
n4:obor
n15:EB
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
5
n4:projekt
n13:LC535 n13:2B06052
n4:rokUplatneniVysledku
n9:2010
n4:tvurceVysledku
Páclová, Daniela Matula, Pavel Vařecha, Miroslav Procházková, Jiřina Kozubek, Michal
n4:zamer
n18:MSM0021622419
n12:organizacniJednotka
14330