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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F10%3A00044700%21RIV11-MSM-14330___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Apart from this function in cell death, AIF also plays largely unknown role in cell life. We used AIF gene silencing using shRNA vectors followed by measurements by flow cytometry and confocal fluorescent microscopy to detect the superoxide production during oxidative phosphorylation in mitochondria. We managed to knock-down the expression of gene AIF by transient or stable transfection in various human cell lines. Interestingly, mitochondrial superoxide production in various cell lines reacted to gene silencing of AIF differently. Cell line U-2 OS transiently and also stably transfected with vector shRNA was found to produce less superoxide in comparison to control U-2 OS cells. Interestingly, HeLa cell line shows opposite results with significantly increased mitochondrial superoxide production in silenced HeLa cells. We are also in process of examination of other healthy and cancer cell. Apart from this function in cell death, AIF also plays largely unknown role in cell life. We used AIF gene silencing using shRNA vectors followed by measurements by flow cytometry and confocal fluorescent microscopy to detect the superoxide production during oxidative phosphorylation in mitochondria. We managed to knock-down the expression of gene AIF by transient or stable transfection in various human cell lines. Interestingly, mitochondrial superoxide production in various cell lines reacted to gene silencing of AIF differently. Cell line U-2 OS transiently and also stably transfected with vector shRNA was found to produce less superoxide in comparison to control U-2 OS cells. Interestingly, HeLa cell line shows opposite results with significantly increased mitochondrial superoxide production in silenced HeLa cells. We are also in process of examination of other healthy and cancer cell.
dcterms:title
Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell lines Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell lines
skos:prefLabel
Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell lines Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell lines
skos:notation
RIV/00216224:14330/10:00044700!RIV11-MSM-14330___
n4:aktivita
n12:P n12:Z
n4:aktivity
P(2B06052), P(LC535), Z(MSM0021622419)
n4:dodaniDat
n15:2011
n4:domaciTvurceVysledku
n14:9465146 n14:1723162 n14:3283038 n14:3021475
n4:druhVysledku
n7:O
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n11:predkladatel
n4:idSjednocenehoVysledku
246017
n4:idVysledku
RIV/00216224:14330/10:00044700
n4:jazykVysledku
n5:eng
n4:klicovaSlova
AIF; shRNA; gene silencing; image analysis; superoxide; mitochondria; oxidative phosphorylation
n4:klicoveSlovo
n9:mitochondria n9:oxidative%20phosphorylation n9:superoxide n9:shRNA n9:AIF n9:image%20analysis n9:gene%20silencing
n4:kontrolniKodProRIV
[3E985774039B]
n4:obor
n10:EB
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
5
n4:projekt
n8:2B06052 n8:LC535
n4:rokUplatneniVysledku
n15:2010
n4:tvurceVysledku
Vařecha, Miroslav Procházková, Jiřina Páclová, Daniela Kozubek, Michal Matula, Pavel
n4:zamer
n18:MSM0021622419
n13:organizacniJednotka
14330