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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F09%3A00048866%21RIV11-GA0-14330___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
In our analyses we combined molecular and computational approaches to understand the mutant p53 function in specific gene regulation via binding to non-canonical DNA structures in chromatin DNA. We used two glioblastoma cell lines U251 (R273H) and Onda11 (R273C) expressing endogenous mutp53 proteins to isolate natural mutant p53 binding sites (mutp53BS) by genome- wide ChIP-cloning. In our computational work, we developed tools for rapid identification of DNA sequences (among the isolated mutp53BS) tending to form non-B structures (hairpins and triplex DNA) as well as for mapping their genomic locations. These sites are frequently localized in the regulatory first introns of genes and are enriched in repetitive elements. Potential to form triplex and cruciform structures was predicted by developed computational tools and detected by enzymatic and chemical probing. In our analyses we combined molecular and computational approaches to understand the mutant p53 function in specific gene regulation via binding to non-canonical DNA structures in chromatin DNA. We used two glioblastoma cell lines U251 (R273H) and Onda11 (R273C) expressing endogenous mutp53 proteins to isolate natural mutant p53 binding sites (mutp53BS) by genome- wide ChIP-cloning. In our computational work, we developed tools for rapid identification of DNA sequences (among the isolated mutp53BS) tending to form non-B structures (hairpins and triplex DNA) as well as for mapping their genomic locations. These sites are frequently localized in the regulatory first introns of genes and are enriched in repetitive elements. Potential to form triplex and cruciform structures was predicted by developed computational tools and detected by enzymatic and chemical probing.
dcterms:title
Recognition of non-canonical DNA structures in genomic DNA sequences by p53 proteins Recognition of non-canonical DNA structures in genomic DNA sequences by p53 proteins
skos:prefLabel
Recognition of non-canonical DNA structures in genomic DNA sequences by p53 proteins Recognition of non-canonical DNA structures in genomic DNA sequences by p53 proteins
skos:notation
RIV/00216224:14330/09:00048866!RIV11-GA0-14330___
n3:aktivita
n5:P
n3:aktivity
P(GA204/08/1560)
n3:dodaniDat
n9:2011
n3:domaciTvurceVysledku
n7:4171373 n7:6759890
n3:druhVysledku
n17:O
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
338373
n3:idVysledku
RIV/00216224:14330/09:00048866
n3:jazykVysledku
n16:eng
n3:klicovaSlova
p53; triplex DNA; DNA-binding; gene regulation
n3:klicoveSlovo
n8:gene%20regulation n8:p53 n8:triplex%20DNA n8:DNA-binding
n3:kontrolniKodProRIV
[B420561C6C19]
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
8
n3:projekt
n11:GA204%2F08%2F1560
n3:rokUplatneniVysledku
n9:2009
n3:tvurceVysledku
Brazdova, Marie Deppert, W. Lexa, Matej Fojta, Miroslav Navrátilová, L. Tichy, V. Kyjovsky, I. Paleček, E.
n6:organizacniJednotka
14330