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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F09%3A00034548%21RIV11-MSM-14330___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to more precisely analyze defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The HL60 cell line was used in the search for a suitable protocol that can be used for clinical samples of CD34+ hematopoietic cells obtained from bone marrow. The goal was to discover the best method of isolating and amplifying RNA from a small number of cells. Our evaluation of various methods and kits available in the market revealed that the combination of RNAqueous Kit (Ambion, USA) for RNA isolation and the SenseAmp Plus Kit (Genisphere, USA) for one-round RNA amplification produced the best results. cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to more precisely analyze defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The HL60 cell line was used in the search for a suitable protocol that can be used for clinical samples of CD34+ hematopoietic cells obtained from bone marrow. The goal was to discover the best method of isolating and amplifying RNA from a small number of cells. Our evaluation of various methods and kits available in the market revealed that the combination of RNAqueous Kit (Ambion, USA) for RNA isolation and the SenseAmp Plus Kit (Genisphere, USA) for one-round RNA amplification produced the best results.
dcterms:title
Microarray analysis using limited amount of cells Microarray analysis using limited amount of cells
skos:prefLabel
Microarray analysis using limited amount of cells Microarray analysis using limited amount of cells
skos:notation
RIV/00216224:14330/09:00034548!RIV11-MSM-14330___
n3:aktivita
n9:P n9:V n9:Z
n3:aktivity
P(2B06052), P(LC535), V, Z(MSM0021622430)
n3:cisloPeriodika
55
n3:dodaniDat
n15:2011
n3:domaciTvurceVysledku
n5:4748891 n5:3021475 n5:4376285 n5:3922014 n5:6986099 n5:2532883 n5:9619658 n5:1248790 n5:8682968
n3:druhVysledku
n7:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
326151
n3:idVysledku
RIV/00216224:14330/09:00034548
n3:jazykVysledku
n12:eng
n3:klicovaSlova
cDNA microarray; RNA isolation; RNA amplifiacation; CD34+ cells
n3:klicoveSlovo
n6:cDNA%20microarray n6:CD34%2B%20cells n6:RNA%20amplifiacation n6:RNA%20isolation
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[1EB31851CE7A]
n3:nazevZdroje
Folia Biologica (Praha)
n3:obor
n14:EB
n3:pocetDomacichTvurcuVysledku
9
n3:pocetTvurcuVysledku
9
n3:projekt
n8:LC535 n8:2B06052
n3:rokUplatneniVysledku
n15:2009
n3:svazekPeriodika
2009
n3:tvurceVysledku
Doubek, Michael Klabusay, Martin Mayer, Jiří Tesařová, Lenka Hrabčáková, Viera Peterková, Martina Krontorád Koutná, Irena Potěšilová, Michaela Kozubek, Michal
n3:wos
000265765100004
n3:zamer
n16:MSM0021622430
s:issn
0015-5500
s:numberOfPages
8
n13:organizacniJednotka
14330