This HTML5 document contains 53 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n12http://localhost/temp/predkladatel/
n19http://linked.opendata.cz/resource/domain/vavai/projekt/
n5http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n17http://linked.opendata.cz/ontology/domain/vavai/
n14http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n8http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n7http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14330%2F09%3A00034277%21RIV10-MSM-14330___/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n16http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n11http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F09%3A00034277%21RIV10-MSM-14330___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
This study combines mRNA and protein analysis using cDNA and antibody microarray techniques, respectively. These create a novel, integrated perspective into cellular molecular profiles. The aims of this study were to establish a reliable way of integrating these two approaches in order to obtain complex molecular profiles of the cell and to find suitable methods to normalize the data obtained using these approaches. Antibody microarray and cDNA microarray techniques were used to study expression alterations in HL-60 cells that were differentiated into granulocytes using all-trans retinoic acid (ATRA). We selected this model to evaluate this combined profiling technique because the expression levels of most of the mRNA and protein species in these cells are not altered; therefore it is easier to track and define those species that are changed. The proteins whose levels were altered included cmyc, c-jun, Pyk2, FAK, PKC, TRF1, NF-kappaB and certain caspase types. This study combines mRNA and protein analysis using cDNA and antibody microarray techniques, respectively. These create a novel, integrated perspective into cellular molecular profiles. The aims of this study were to establish a reliable way of integrating these two approaches in order to obtain complex molecular profiles of the cell and to find suitable methods to normalize the data obtained using these approaches. Antibody microarray and cDNA microarray techniques were used to study expression alterations in HL-60 cells that were differentiated into granulocytes using all-trans retinoic acid (ATRA). We selected this model to evaluate this combined profiling technique because the expression levels of most of the mRNA and protein species in these cells are not altered; therefore it is easier to track and define those species that are changed. The proteins whose levels were altered included cmyc, c-jun, Pyk2, FAK, PKC, TRF1, NF-kappaB and certain caspase types.
dcterms:title
Combination of mRNA and protein microarray analysis in complex cell profiling Combination of mRNA and protein microarray analysis in complex cell profiling
skos:prefLabel
Combination of mRNA and protein microarray analysis in complex cell profiling Combination of mRNA and protein microarray analysis in complex cell profiling
skos:notation
RIV/00216224:14330/09:00034277!RIV10-MSM-14330___
n3:aktivita
n4:Z n4:P
n3:aktivity
P(2B06052), Z(MSM0021622430)
n3:cisloPeriodika
56
n3:dodaniDat
n11:2010
n3:domaciTvurceVysledku
n5:3021475 n5:4748891 n5:3809404 n5:3922014 n5:8054142 n5:8836086 n5:2346257
n3:druhVysledku
n15:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
307527
n3:idVysledku
RIV/00216224:14330/09:00034277
n3:jazykVysledku
n16:eng
n3:klicovaSlova
microarray; cell profiling; protein expression; mRNA expression; HL-60
n3:klicoveSlovo
n8:microarray n8:protein%20expression n8:cell%20profiling n8:mRNA%20expression n8:HL-60
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[9E51CE8BA297]
n3:nazevZdroje
Neoplasma
n3:obor
n18:EB
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:projekt
n19:2B06052
n3:rokUplatneniVysledku
n11:2009
n3:svazekPeriodika
2/2009
n3:tvurceVysledku
Krontorád, Petr Kozubek, Michal Peterková, Martina Stejskal, Stanislav Krontorád Koutná, Irena Ručka, Zdeněk Šimara, Pavel
n3:zamer
n14:MSM0021622430
s:issn
0028-2685
s:numberOfPages
9
n12:organizacniJednotka
14330