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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F08%3A00027029%21RIV10-MSM-14330___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to analyze more precisely defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The goal was to find the best method of isolating and amplifying RNA from a small number of cells. We used the HL60 cell line in the search for a suitable protocol that could be applied to clinical samples of CD34+ hematopoietic cells. These cells make up only a very small population in bone marrow (1- 4% of all cells). cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to analyze more precisely defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The goal was to find the best method of isolating and amplifying RNA from a small number of cells. We used the HL60 cell line in the search for a suitable protocol that could be applied to clinical samples of CD34+ hematopoietic cells. These cells make up only a very small population in bone marrow (1- 4% of all cells).
dcterms:title
cDNA Microarray: Gene Expression Profiling of Small Cell Populations cDNA Microarray: Gene Expression Profiling of Small Cell Populations
skos:prefLabel
cDNA Microarray: Gene Expression Profiling of Small Cell Populations cDNA Microarray: Gene Expression Profiling of Small Cell Populations
skos:notation
RIV/00216224:14330/08:00027029!RIV10-MSM-14330___
n5:aktivita
n14:Z n14:P
n5:aktivity
P(2B06052), P(LC535), Z(MSM0021622430)
n5:dodaniDat
n11:2010
n5:domaciTvurceVysledku
n7:2532883 n7:8836086 n7:9619658 n7:8682968 n7:6986099 n7:3922014 n7:4748891
n5:druhVysledku
n16:O
n5:duvernostUdaju
n13:S
n5:entitaPredkladatele
n17:predkladatel
n5:idSjednocenehoVysledku
358970
n5:idVysledku
RIV/00216224:14330/08:00027029
n5:jazykVysledku
n18:eng
n5:klicovaSlova
cDNA microarray; RNA isolation; RNA amplifiacation; CD34+ cells
n5:klicoveSlovo
n8:cDNA%20microarray n8:CD34%2B%20cells n8:RNA%20isolation n8:RNA%20amplifiacation
n5:kontrolniKodProRIV
[40A2CA0B2606]
n5:obor
n9:EB
n5:pocetDomacichTvurcuVysledku
7
n5:pocetTvurcuVysledku
7
n5:projekt
n10:LC535 n10:2B06052
n5:rokUplatneniVysledku
n11:2008
n5:tvurceVysledku
Hrabčáková, Viera Klabusay, Martin Tesařová, Lenka Ručka, Zdeněk Potěšilová, Michaela Peterková, Martina Krontorád Koutná, Irena
n5:zamer
n15:MSM0021622430
n12:organizacniJednotka
14330