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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F08%3A00026068%21RIV11-MSM-14330___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in living cells using both single-cell colocalization image analysis and nuclear translocation image analysis. Image data were acquired using automated fluorescent microscope Zeiss Axiovert 200M with spinning disc confocal unit Yokogawa CSU-10. We confirmed the mitochondrial localization of endonuclease G and AIF by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF. We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in living cells using both single-cell colocalization image analysis and nuclear translocation image analysis. Image data were acquired using automated fluorescent microscope Zeiss Axiovert 200M with spinning disc confocal unit Yokogawa CSU-10. We confirmed the mitochondrial localization of endonuclease G and AIF by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF.
dcterms:title
Image analysis of localization and translocation of apoptotic proteins in living cells Image analysis of localization and translocation of apoptotic proteins in living cells
skos:prefLabel
Image analysis of localization and translocation of apoptotic proteins in living cells Image analysis of localization and translocation of apoptotic proteins in living cells
skos:notation
RIV/00216224:14330/08:00026068!RIV11-MSM-14330___
n3:aktivita
n16:Z n16:P
n3:aktivity
P(LC535), Z(MSM0021622419)
n3:dodaniDat
n15:2011
n3:domaciTvurceVysledku
n4:3021475 n4:9928758 n4:9238174 n4:1723162 n4:1270494
n3:druhVysledku
n17:O
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
371406
n3:idVysledku
RIV/00216224:14330/08:00026068
n3:jazykVysledku
n9:eng
n3:klicovaSlova
AIF; endonuclease G; AMID; living cells; colocalization; image analysis
n3:klicoveSlovo
n8:living%20cells n8:endonuclease%20G n8:AMID n8:image%20analysis n8:AIF n8:colocalization
n3:kontrolniKodProRIV
[E788C8CACD2A]
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n10:LC535
n3:rokUplatneniVysledku
n15:2008
n3:tvurceVysledku
Amrichová, Jana Vařecha, Miroslav Zimmermann, Michal Ulman, Vladimír Kozubek, Michal
n3:zamer
n18:MSM0021622419
n13:organizacniJednotka
14330