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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F06%3A00033148%21RIV11-MSM-14330___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
The successful development of non-invasive quantitative visualization techniques for live cell imaging have led to the development of suitable hardware and software for the acquisition and processing of multidimensional image data. Confocal spinning disk systems (based either on a classical Nipkow disk or on the microlens principle) are especially suitable for live cell imaging thanks to high acquisition speed (parallel imaging of thousands of points), high quality image detection (up to 90% quantum efficiency at negligible noise levels with state-of-the-art cameras), low photobleaching and low phototoxicity. Lately, we have been working on the optimization and automation of image acquisition and processing for this type of microscopy. This poster presents full description of our hardware set-up and several applications from live cell imaging that are carried out on this set-up in our laboratory. The successful development of non-invasive quantitative visualization techniques for live cell imaging have led to the development of suitable hardware and software for the acquisition and processing of multidimensional image data. Confocal spinning disk systems (based either on a classical Nipkow disk or on the microlens principle) are especially suitable for live cell imaging thanks to high acquisition speed (parallel imaging of thousands of points), high quality image detection (up to 90% quantum efficiency at negligible noise levels with state-of-the-art cameras), low photobleaching and low phototoxicity. Lately, we have been working on the optimization and automation of image acquisition and processing for this type of microscopy. This poster presents full description of our hardware set-up and several applications from live cell imaging that are carried out on this set-up in our laboratory.
dcterms:title
Automated confocal live cell microscopy based on spinning disks Automated confocal live cell microscopy based on spinning disks
skos:prefLabel
Automated confocal live cell microscopy based on spinning disks Automated confocal live cell microscopy based on spinning disks
skos:notation
RIV/00216224:14330/06:00033148!RIV11-MSM-14330___
n4:aktivita
n5:P n5:Z
n4:aktivity
P(LC535), Z(MSM0021622419)
n4:dodaniDat
n10:2011
n4:domaciTvurceVysledku
n7:1723162 n7:1270494 n7:8491313 n7:5014409 n7:8544212 n7:3021475 n7:9465146
n4:druhVysledku
n8:O
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
466378
n4:idVysledku
RIV/00216224:14330/06:00033148
n4:jazykVysledku
n13:eng
n4:klicovaSlova
confocal microscopy; image processing
n4:klicoveSlovo
n15:image%20processing n15:confocal%20microscopy
n4:kontrolniKodProRIV
[19146B1A027D]
n4:obor
n12:JD
n4:pocetDomacichTvurcuVysledku
7
n4:pocetTvurcuVysledku
8
n4:projekt
n16:LC535
n4:rokUplatneniVysledku
n10:2006
n4:tvurceVysledku
Matula, Pavel Kozubek, Stanislav Kozubek, Michal Ondřej, Vladan Lukášová, Emilie Amrichová, Jana Vařecha, Miroslav Matula, Petr
n4:zamer
n6:MSM0021622419
n18:organizacniJednotka
14330