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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F05%3A00013294%21RIV11-GA0-14330___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Our project is focused on study of mitochondrial apoptogenic proteins released during apoptosis and associated with caspase-independent nuclear chromatin condensation and degradation. We introduce our automated 2D/3D/4D/FRET high-resolution image data acquisition and analysis system developed to visualize and quantify fluorescence signals in fixed and living cells. For our studies, we utilize the fluorescent proteins and various low-toxicity cell permeable fluorescent probes that make it possible to conduct the non-invasive quantitative visualization in living cells. In our experiments, cells are stably or transiently transfected or cotransfected by lipofection with DNA plasmids coding fusion proteins of mitochondrial apoptogenic proteins (cytochrom c, AIF, amid, and endonuclease g) with one of fluorescence proteins (EGFP, EYFP, or t-HcRed). Our project is focused on study of mitochondrial apoptogenic proteins released during apoptosis and associated with caspase-independent nuclear chromatin condensation and degradation. We introduce our automated 2D/3D/4D/FRET high-resolution image data acquisition and analysis system developed to visualize and quantify fluorescence signals in fixed and living cells. For our studies, we utilize the fluorescent proteins and various low-toxicity cell permeable fluorescent probes that make it possible to conduct the non-invasive quantitative visualization in living cells. In our experiments, cells are stably or transiently transfected or cotransfected by lipofection with DNA plasmids coding fusion proteins of mitochondrial apoptogenic proteins (cytochrom c, AIF, amid, and endonuclease g) with one of fluorescence proteins (EGFP, EYFP, or t-HcRed).
dcterms:title
Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells. Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
skos:prefLabel
Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells. Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
skos:notation
RIV/00216224:14330/05:00013294!RIV11-GA0-14330___
n3:aktivita
n9:P n9:Z
n3:aktivity
P(GA202/04/0907), P(GP204/03/D031), P(GP204/05/P090), Z(MSM0021622419)
n3:dodaniDat
n4:2011
n3:domaciTvurceVysledku
n10:8544212 n10:5930731 n10:1723162 n10:3021475 n10:5014409 n10:8491313 n10:1270494 n10:9238174
n3:druhVysledku
n18:O
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
524242
n3:idVysledku
RIV/00216224:14330/05:00013294
n3:jazykVysledku
n17:eng
n3:klicovaSlova
living cells; GFP; fluorescent protein; FRET; fluorescent microscopy
n3:klicoveSlovo
n5:living%20cells n5:FRET n5:GFP n5:fluorescent%20protein n5:fluorescent%20microscopy
n3:kontrolniKodProRIV
[E9C605BDEDEA]
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
8
n3:projekt
n6:GP204%2F05%2FP090 n6:GA202%2F04%2F0907 n6:GP204%2F03%2FD031
n3:rokUplatneniVysledku
n4:2005
n3:tvurceVysledku
Ulman, Vladimír Kozubek, Michal Kozubek, Stanislav Lukášová, Emilie Ondřej, Vladan Amrichová, Jana Vařecha, Miroslav Matula, Petr
n3:zamer
n16:MSM0021622419
n8:organizacniJednotka
14330