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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F03%3A00008544%21RIV09-MSM-14330___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Micro axial tomography is a challenging technique in microscopy which improves quantitative imaging especially in cytogenetic applications by means of defined sample rotation under the microscope objective. The advantage of micro-axial tomography is an effective improvement of the precision of distance measurements between point-like objects. Under certain circumstances, the effective (3D) resolution can be improved by optimized acquisition depending on subsequent, multi-perspective image recording of the same objects followed by reconstruction methods. This requires, however, a very precise alignment of the tilted views. We present a novel feature-based image alignment method with a precision better than the full width at half maximum of the point spread function. The features are the positions (centres of gravity) of all fluorescent objects observed in the images (e.g. cell nuclei, fluorescent signals inside cell nuclei, fluorescent beads, etc.). Thus, real alignment precision depends on the localiz Micro axial tomography is a challenging technique in microscopy which improves quantitative imaging especially in cytogenetic applications by means of defined sample rotation under the microscope objective. The advantage of micro-axial tomography is an effective improvement of the precision of distance measurements between point-like objects. Under certain circumstances, the effective (3D) resolution can be improved by optimized acquisition depending on subsequent, multi-perspective image recording of the same objects followed by reconstruction methods. This requires, however, a very precise alignment of the tilted views. We present a novel feature-based image alignment method with a precision better than the full width at half maximum of the point spread function. The features are the positions (centres of gravity) of all fluorescent objects observed in the images (e.g. cell nuclei, fluorescent signals inside cell nuclei, fluorescent beads, etc.). Thus, real alignment precision depends on the localiz Micro axial tomography is a challenging technique in microscopy which improves quantitative imaging especially in cytogenetic applications by means of defined sample rotation under the microscope objective. The advantage of micro-axial tomography is an effective improvement of the precision of distance measurements between point-like objects. Under certain circumstances, the effective (3D) resolution can be improved by optimized acquisition depending on subsequent, multi-perspective image recording of the same objects followed by reconstruction methods. This requires, however, a very precise alignment of the tilted views. We present a novel feature-based image alignment method with a precision better than the full width at half maximum of the point spread function. The features are the positions (centres of gravity) of all fluorescent objects observed in the images (e.g. cell nuclei, fluorescent signals inside cell nuclei, fluorescent beads, etc.). Thus, real alignment precision depends on the localiz
dcterms:title
Precise 3D image alignment in micro-axial tomography Precise 3D image alignment in micro-axial tomography Precise 3D image alignment in micro-axial tomography
skos:prefLabel
Precise 3D image alignment in micro-axial tomography Precise 3D image alignment in micro-axial tomography Precise 3D image alignment in micro-axial tomography
skos:notation
RIV/00216224:14330/03:00008544!RIV09-MSM-14330___
n3:aktivita
n4:P n4:Z
n3:aktivity
P(IBS5004010), Z(MSM 143300002)
n3:cisloPeriodika
2
n3:dodaniDat
n5:2009
n3:domaciTvurceVysledku
n15:3021475 n15:8544212
n3:druhVysledku
n6:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
622459
n3:idVysledku
RIV/00216224:14330/03:00008544
n3:jazykVysledku
n9:eng
n3:klicovaSlova
assignment; image fusion; image registration; Kuhn-Munkres algorithm; micro-axial tomography; resolution improvement
n3:klicoveSlovo
n7:Kuhn-Munkres%20algorithm n7:image%20fusion n7:assignment n7:image%20registration n7:resolution%20improvement n7:micro-axial%20tomography
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[1C4BFCA40BDF]
n3:nazevZdroje
Journal of Microscopy
n3:obor
n18:JD
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n19:IBS5004010
n3:rokUplatneniVysledku
n5:2003
n3:svazekPeriodika
209
n3:tvurceVysledku
Matula, Petr Kozubek, Michal Hausmann, Michael Staier, Florian
n3:zamer
n10:MSM%20143300002
s:issn
0022-2720
s:numberOfPages
17
n8:organizacniJednotka
14330