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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F03%3A00008243%21RIV11-AV0-14330___
rdf:type
n20:Vysledek skos:Concept
dcterms:description
The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL In comparison with the control, an increased number of centromeric FISH signals were observed in prolonged confluence treated K-562 cells induced to apoptosis. The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL In comparison with the control, an increased number of centromeric FISH signals were observed in prolonged confluence treated K-562 cells induced to apoptosis.
dcterms:title
Architecture of chromosomal territories in apoptotic cells Architecture of chromosomal territories in apoptotic cells
skos:prefLabel
Architecture of chromosomal territories in apoptotic cells Architecture of chromosomal territories in apoptotic cells
skos:notation
RIV/00216224:14330/03:00008243!RIV11-AV0-14330___
n3:aktivita
n6:P n6:Z
n3:aktivity
P(GA301/01/0186), P(IAB5004102), P(IBS5004010), Z(MSM 143300002)
n3:dodaniDat
n14:2011
n3:domaciTvurceVysledku
n5:8491313 n5:9465146 n5:4873130
n3:druhVysledku
n19:D
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
599033
n3:idVysledku
RIV/00216224:14330/03:00008243
n3:jazykVysledku
n21:eng
n3:klicovaSlova
human genome structure
n3:klicoveSlovo
n7:human%20genome%20structure
n3:kontrolniKodProRIV
[63F5EC957019]
n3:mistoKonaniAkce
Hlohovec,Czech Republic
n3:mistoVydani
Brno
n3:nazevZdroje
Biophysics of the Genome
n3:obor
n4:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n9:IBS5004010 n9:GA301%2F01%2F0186 n9:IAB5004102
n3:rokUplatneniVysledku
n14:2003
n3:tvurceVysledku
Bártová, Eva Palša, Stanislav Matula, Pavel Kozubek, Stanislav Fojtová, Miloslava Souček, Karel
n3:typAkce
n22:EUR
n3:zahajeniAkce
2003-01-01+01:00
n3:zamer
n17:MSM%20143300002
s:numberOfPages
10
n18:hasPublisher
Masaryk University
n16:isbn
80-210-3226-X
n13:organizacniJednotka
14330