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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F02%3A00006556%21RIV11-AV0-14330___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Topography of three genetic elements - dystrophin (dmd) exons 5-7 (E-1), 46-47 (E-2) and centromere of chromosome X (N-x) were studied relative to cell nuclei and to chromosome X territories of spatially fixed human lymphocytes. Repeated three-dimensional (3D) dual color fluorescence in situ hybridization combined with high-resolution cytometry was used. In addition, the nuclear location of fluorescence weight centers (FWC), spatial volume, and maximal area per one section of chromosome-X territories were investigated. The larger (X-L) and smaller (X-s) homologous X-chromosomes were distinguished for each nucleus according to the 3D volume of their territories. The distributions of the [center of nucleus]-to-[genetic element] distances (radial distributions) of dmd exons E-1, E-2, centromere N-x and FWC were very similar for both homologous X-chromosomes of female lymphocytes as well as for the chromosome X of the human male. Topography of three genetic elements - dystrophin (dmd) exons 5-7 (E-1), 46-47 (E-2) and centromere of chromosome X (N-x) were studied relative to cell nuclei and to chromosome X territories of spatially fixed human lymphocytes. Repeated three-dimensional (3D) dual color fluorescence in situ hybridization combined with high-resolution cytometry was used. In addition, the nuclear location of fluorescence weight centers (FWC), spatial volume, and maximal area per one section of chromosome-X territories were investigated. The larger (X-L) and smaller (X-s) homologous X-chromosomes were distinguished for each nucleus according to the 3D volume of their territories. The distributions of the [center of nucleus]-to-[genetic element] distances (radial distributions) of dmd exons E-1, E-2, centromere N-x and FWC were very similar for both homologous X-chromosomes of female lymphocytes as well as for the chromosome X of the human male.
dcterms:title
Topography of genetic elements of X-chromosome relative to the cell nucleus and to the chromosome X territory determined for human lymphocytes Topography of genetic elements of X-chromosome relative to the cell nucleus and to the chromosome X territory determined for human lymphocytes
skos:prefLabel
Topography of genetic elements of X-chromosome relative to the cell nucleus and to the chromosome X territory determined for human lymphocytes Topography of genetic elements of X-chromosome relative to the cell nucleus and to the chromosome X territory determined for human lymphocytes
skos:notation
RIV/00216224:14330/02:00006556!RIV11-AV0-14330___
n3:aktivita
n11:Z n11:P
n3:aktivity
P(GA202/01/0197), P(GA301/01/0186), P(IBS5004010), P(NC5955), Z(AV0Z5004920)
n3:cisloPeriodika
1
n3:dodaniDat
n8:2011
n3:domaciTvurceVysledku
n16:5014409 n16:5810655 n16:8491313 n16:3021475
n3:druhVysledku
n4:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
667114
n3:idVysledku
RIV/00216224:14330/02:00006556
n3:jazykVysledku
n17:eng
n3:klicovaSlova
nuclear architecture; chromosome X inactivation; three dimensional fluorescence in situ hybridization; confocal microscopy; chromatin; condensation
n3:klicoveSlovo
n5:chromatin n5:nuclear%20architecture n5:chromosome%20X%20inactivation n5:confocal%20microscopy n5:three%20dimensional%20fluorescence%20in%20situ%20hybridization n5:condensation
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[115EC907237C]
n3:nazevZdroje
Gene
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n7:GA202%2F01%2F0197 n7:NC5955 n7:GA301%2F01%2F0186 n7:IBS5004010
n3:rokUplatneniVysledku
n8:2002
n3:svazekPeriodika
292
n3:tvurceVysledku
Kozubek, Stanislav Lukášová, Emilie Kozubek, Michal Falk, Martin
n3:zamer
n12:AV0Z5004920
s:issn
0378-1119
s:numberOfPages
12
n6:organizacniJednotka
14330