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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F02%3A00005604%21RIV08-AV0-14330___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Using a novel system for expressing ipt gene from Agrobacterium tumefaciens in tobacco (Nicotiana tabacum L., cv. Petit Havana SR1), we were able to grow seedlings and teratoma-like tissue with increased content of cytokinins. This material enabled us to investigate new regulatory aspects of nitrate reduction. We had grown control plants and plants with elevated cytokinins on MS media with or without nitrate and benzyladenine (BA). We determined in vitro nitrate reductase (E.C. 1.6.6.1) activity (NRA) in this plant material. Initially, we found that ipt-expressing plants always displayed lowered levels of NRA when compared to wild-type SR1 plants. We determined that long-term exposure of tobacco plants and tissue to cytokinins caused up to 60% decrease in NRA. Exposure to 40 mM nitrate was able to induce the activity in such plants 3-fold, increasing the activity in SR1 plants more than 5-fold. We were able to restore wild-type levels of NRA in ipt-expressing plants by simultaneous induction of NR wit Using a novel system for expressing ipt gene from Agrobacterium tumefaciens in tobacco (Nicotiana tabacum L., cv. Petit Havana SR1), we were able to grow seedlings and teratoma-like tissue with increased content of cytokinins. This material enabled us to investigate new regulatory aspects of nitrate reduction. We had grown control plants and plants with elevated cytokinins on MS media with or without nitrate and benzyladenine (BA). We determined in vitro nitrate reductase (E.C. 1.6.6.1) activity (NRA) in this plant material. Initially, we found that ipt-expressing plants always displayed lowered levels of NRA when compared to wild-type SR1 plants. We determined that long-term exposure of tobacco plants and tissue to cytokinins caused up to 60% decrease in NRA. Exposure to 40 mM nitrate was able to induce the activity in such plants 3-fold, increasing the activity in SR1 plants more than 5-fold. We were able to restore wild-type levels of NRA in ipt-expressing plants by simultaneous induction of NR wit Using a novel system for expressing ipt gene from Agrobacterium tumefaciens in tobacco (Nicotiana tabacum L., cv. Petit Havana SR1), we were able to grow seedlings and teratoma-like tissue with increased content of cytokinins. This material enabled us to investigate new regulatory aspects of nitrate reduction. We had grown control plants and plants with elevated cytokinins on MS media with or without nitrate and benzyladenine (BA). We determined in vitro nitrate reductase (E.C. 1.6.6.1) activity (NRA) in this plant material. Initially, we found that ipt-expressing plants always displayed lowered levels of NRA when compared to wild-type SR1 plants. We determined that long-term exposure of tobacco plants and tissue to cytokinins caused up to 60% decrease in NRA. Exposure to 40 mM nitrate was able to induce the activity in such plants 3-fold, increasing the activity in SR1 plants more than 5-fold. We were able to restore wild-type levels of NRA in ipt-expressing plants by simultaneous induction of NR wit
dcterms:title
Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine
skos:prefLabel
Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine
skos:notation
RIV/00216224:14330/02:00005604!RIV08-AV0-14330___
n3:strany
284
n3:aktivita
n5:P n5:Z
n3:aktivity
P(IAA5004001), P(VS96096), Z(MSM 143100008)
n3:cisloPeriodika
2
n3:dodaniDat
n11:2008
n3:domaciTvurceVysledku
n6:9071105 n6:6759890 n6:9223428
n3:druhVysledku
n17:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
649178
n3:idVysledku
RIV/00216224:14330/02:00005604
n3:jazykVysledku
n12:eng
n3:klicovaSlova
cytokinins; Dianthus caryophyllus; 4-pu-30; thidiazuron; photosystem II; chlorophyll fluorescence; delayed fluorescence; heat-induced changes; gas-exchange; photosynthetic response; cytokinin activity; high-temperature; plant-growth; in-vitro; accumulation; adaptation; cotyledons.
n3:klicoveSlovo
n4:plant-growth n4:gas-exchange n4:thidiazuron n4:high-temperature n4:cytokinins n4:chlorophyll%20fluorescence n4:photosystem%20II n4:cotyledons. n4:4-pu-30 n4:accumulation n4:Dianthus%20caryophyllus n4:photosynthetic%20response n4:delayed%20fluorescence n4:in-vitro n4:cytokinin%20activity n4:heat-induced%20changes n4:adaptation
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[2C93336C80BD]
n3:nazevZdroje
PHYSIOLOGIA PLANTARUM
n3:obor
n18:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n10:IAA5004001 n10:VS96096
n3:rokUplatneniVysledku
n11:2002
n3:svazekPeriodika
115
n3:tvurceVysledku
Brzobohatý, Břetislav Lexa, Matej Genkov, Todor Nikolaev
n3:zamer
n19:MSM%20143100008
s:issn
0031-9317
s:numberOfPages
7
n15:organizacniJednotka
14330