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Statements

Subject Item
n2:RIV%2F00216224%3A14330%2F01%3A00004339%21RIV09-GA0-14330___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
The recently developed technique of high-resolution cytometry (HRCM) enables automated acquisition and analysis of FISH stained cell nuclei using wide-field fluorescence microscopy. The method has now been extended to confocal imaging and offers the opportunity to combine the advantages of confocal and wide-field modes. Using the combined confocal and wide-field HRCM technique, it is possible to take advantage of both imaging modes. Images of some dyes (such as small hybridization dots or counterstain images of individual interphase nuclei) do not require confocal quality and can be acquired quickly in wide-field mode. On the contrary, images of other dyes (such as chromosome territories or counterstain images of cells in tissues) do require improved quality and are acquired in confocal mode. The dual-mode approach is 2-3 times faster compared with the single-mode confocal approach and the spectrum of its applications is much broader compared with both single-mode confocal and single-mode wide-field s The recently developed technique of high-resolution cytometry (HRCM) enables automated acquisition and analysis of FISH stained cell nuclei using wide-field fluorescence microscopy. The method has now been extended to confocal imaging and offers the opportunity to combine the advantages of confocal and wide-field modes. Using the combined confocal and wide-field HRCM technique, it is possible to take advantage of both imaging modes. Images of some dyes (such as small hybridization dots or counterstain images of individual interphase nuclei) do not require confocal quality and can be acquired quickly in wide-field mode. On the contrary, images of other dyes (such as chromosome territories or counterstain images of cells in tissues) do require improved quality and are acquired in confocal mode. The dual-mode approach is 2-3 times faster compared with the single-mode confocal approach and the spectrum of its applications is much broader compared with both single-mode confocal and single-mode wide-field s The recently developed technique of high-resolution cytometry (HRCM) enables automated acquisition and analysis of FISH stained cell nuclei using wide-field fluorescence microscopy. The method has now been extended to confocal imaging and offers the opportunity to combine the advantages of confocal and wide-field modes. Using the combined confocal and wide-field HRCM technique, it is possible to take advantage of both imaging modes. Images of some dyes (such as small hybridization dots or counterstain images of individual interphase nuclei) do not require confocal quality and can be acquired quickly in wide-field mode. On the contrary, images of other dyes (such as chromosome territories or counterstain images of cells in tissues) do require improved quality and are acquired in confocal mode. The dual-mode approach is 2-3 times faster compared with the single-mode confocal approach and the spectrum of its applications is much broader compared with both single-mode confocal and single-mode wide-field s
dcterms:title
Combined confocal and wide-field high-resolution cytometry of FISH-stained cells Combined confocal and wide-field high-resolution cytometry of FISH-stained cells Combined confocal and wide-field high-resolution cytometry of FISH-stained cells
skos:prefLabel
Combined confocal and wide-field high-resolution cytometry of FISH-stained cells Combined confocal and wide-field high-resolution cytometry of FISH-stained cells Combined confocal and wide-field high-resolution cytometry of FISH-stained cells
skos:notation
RIV/00216224:14330/01:00004339!RIV09-GA0-14330___
n3:aktivita
n12:Z n12:P
n3:aktivity
P(GA202/99/P008), P(IBS5004010), P(VS97031), Z(MSM 143300002)
n3:cisloPeriodika
1
n3:dodaniDat
n13:2009
n3:domaciTvurceVysledku
n11:4873130 n11:4092163 n11:8491313 n11:4748891 n11:5881684 n11:5014409 n11:8544212 n11:3021475 n11:8652589 n11:9465146
n3:druhVysledku
n19:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
675877
n3:idVysledku
RIV/00216224:14330/01:00004339
n3:jazykVysledku
n15:eng
n3:klicovaSlova
high-resolution cytometry; fluorescence in situ hybridization; interphase nuclei; fluorescence microscopy; automated microscopy; image analysis; 3-D analysis
n3:klicoveSlovo
n10:fluorescence%20in%20situ%20hybridization n10:interphase%20nuclei n10:automated%20microscopy n10:high-resolution%20cytometry n10:image%20analysis n10:fluorescence%20microscopy n10:3-D%20analysis
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[7DC516A6F0E7]
n3:nazevZdroje
Cytometry
n3:obor
n14:JD
n3:pocetDomacichTvurcuVysledku
10
n3:pocetTvurcuVysledku
10
n3:projekt
n4:GA202%2F99%2FP008 n4:IBS5004010 n4:VS97031
n3:rokUplatneniVysledku
n13:2001
n3:svazekPeriodika
45
n3:tvurceVysledku
Skalníková, Magdalena Kozubek, Stanislav Gaňová, Alena Koutná, Irena Bártová, Eva Gajdušková, Pavla Matula, Petr Matula, Pavel Kozubek, Michal Lukášová, Emilie
n3:zamer
n9:MSM%20143300002
s:issn
0196-4763
s:numberOfPages
12
n8:organizacniJednotka
14330