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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F14%3A00074241%21RIV15-MSM-14310___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
PA-IIL lectin from Pseudomonas aeruginosa shows a unique binding mode among bacterial lectins because it binds saccharides through two calcium cations. Its features make this protein a good candidate for tuning its affinity and specificity. Two amino acids responsible for sugar binding were chosen for targeted-random mutagenesis. If this approach is used, it is necessary to utilize high-throughput screening of mutant libraries for identification of mutants with interestingly modified specificity and/or affinity. PA-IIL lectin from Pseudomonas aeruginosa shows a unique binding mode among bacterial lectins because it binds saccharides through two calcium cations. Its features make this protein a good candidate for tuning its affinity and specificity. Two amino acids responsible for sugar binding were chosen for targeted-random mutagenesis. If this approach is used, it is necessary to utilize high-throughput screening of mutant libraries for identification of mutants with interestingly modified specificity and/or affinity.
dcterms:title
Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library
skos:prefLabel
Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library
skos:notation
RIV/00216224:14310/14:00074241!RIV15-MSM-14310___
n4:aktivita
n9:S n9:P
n4:aktivity
P(GA13-25401S), S
n4:dodaniDat
n6:2015
n4:domaciTvurceVysledku
n11:6666477 n11:5616697 n11:5522064
n4:druhVysledku
n12:O
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
49318
n4:idVysledku
RIV/00216224:14310/14:00074241
n4:jazykVysledku
n8:eng
n4:klicovaSlova
lectins; PA-IIL; Pseudomonas aeruginosa; targeted-random mutagenesis; high-throughput screening; mutant library
n4:klicoveSlovo
n5:high-throughput%20screening n5:PA-IIL n5:targeted-random%20mutagenesis n5:lectins n5:mutant%20library n5:Pseudomonas%20aeruginosa
n4:kontrolniKodProRIV
[6789249D2788]
n4:obor
n7:CE
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
3
n4:projekt
n16:GA13-25401S
n4:rokUplatneniVysledku
n6:2014
n4:tvurceVysledku
Wimmerová, Michaela Mrázková, Jana Pokorná, Martina
n15:organizacniJednotka
14310