This HTML5 document contains 52 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n10http://localhost/temp/predkladatel/
n8http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n4http://linked.opendata.cz/resource/domain/vavai/projekt/
n9http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n7http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n15http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14310%2F14%3A00074201%21RIV15-MSM-14310___/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n16http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n19http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F14%3A00074201%21RIV15-MSM-14310___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid (aa) residues, exhibit only seven amino acid residue differences between them, but LinBMI´s catalytic performance towards beta-hexachlorocyclohexane (beta-HCH) is considerably higher than LinBUT´s. To elucidate the molecular basis governing this difference, intermediate mutants between LinBUT and LinBMI were constructed and kinetically characterized. The activities of LinBUT-based mutants gradually increased by cumulative mutations into LinBUT, and the effects of the individual amino acid substitutions depended on combination with other mutations. These results indicated that LinBUT´s b-HCH degradation activity can be enhanced in a stepwise manner by the accumulation of point mutations. Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid (aa) residues, exhibit only seven amino acid residue differences between them, but LinBMI´s catalytic performance towards beta-hexachlorocyclohexane (beta-HCH) is considerably higher than LinBUT´s. To elucidate the molecular basis governing this difference, intermediate mutants between LinBUT and LinBMI were constructed and kinetically characterized. The activities of LinBUT-based mutants gradually increased by cumulative mutations into LinBUT, and the effects of the individual amino acid substitutions depended on combination with other mutations. These results indicated that LinBUT´s b-HCH degradation activity can be enhanced in a stepwise manner by the accumulation of point mutations.
dcterms:title
Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane.
skos:prefLabel
Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane.
skos:notation
RIV/00216224:14310/14:00074201!RIV15-MSM-14310___
n3:aktivita
n13:I n13:P
n3:aktivity
I, P(GAP503/12/0572), P(LO1214)
n3:cisloPeriodika
72
n3:dodaniDat
n19:2015
n3:domaciTvurceVysledku
n8:1030175 n8:3599019
n3:druhVysledku
n17:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
47494
n3:idVysledku
RIV/00216224:14310/14:00074201
n3:jazykVysledku
n16:eng
n3:klicovaSlova
beta -Hexachlorocyclohexane; Xenobiotics; Biodegradation; Haloalkane dehalogenase; Protein evolution
n3:klicoveSlovo
n5:Protein%20evolution n5:Haloalkane%20dehalogenase n5:beta%20-Hexachlorocyclohexane n5:Biodegradation n5:Xenobiotics
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[BCFAF4555C5D]
n3:nazevZdroje
Applied Microbiology and Biotechnology Express
n3:obor
n12:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
10
n3:projekt
n4:LO1214 n4:GAP503%2F12%2F0572
n3:rokUplatneniVysledku
n19:2014
n3:svazekPeriodika
4
n3:tvurceVysledku
Ohtsubo, Y. Damborský, Jiří Nikawadori, Y. Tsuda, M. Ishitsuka, M. Nagata, Y. Tanaka, H. Prokop, Zbyněk Moriuchi, R. Ito, M.
s:issn
2191-0855
s:numberOfPages
10
n7:doi
10.1186/s13568-014-0072-5
n10:organizacniJednotka
14310