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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F14%3A00073314%21RIV15-MSM-14310___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
The emergence and alarming increase of multiresistant strains of Staphylococcus aureus emphasizes the need for new and innovative antimicrobial strategies. Polyvalent bacteriophages from the family Myoviridae, sub-family Spounavirinae of genus Twort-like phages that infect S. aureus have potential for use in phage therapy. In this study we characterized genomic sequences of the polyvalent staphylococcal phage 812 and its spontaneous host-range mutants selected on non-susceptible S. aureus strains. The wild type phage was shown to lyse up to 65%, whereas host-range mutants up to 95% of S. aureus strains under study. Genomic DNAs of five phages were sequenced using 454 Genome Sequencer Junior (Roche). Bacteriophage DNA sequences were assembled by software GS De novo Assembler and GS Reference Mapper (Roche). Genome of phage K was chosen as the reference. The emergence and alarming increase of multiresistant strains of Staphylococcus aureus emphasizes the need for new and innovative antimicrobial strategies. Polyvalent bacteriophages from the family Myoviridae, sub-family Spounavirinae of genus Twort-like phages that infect S. aureus have potential for use in phage therapy. In this study we characterized genomic sequences of the polyvalent staphylococcal phage 812 and its spontaneous host-range mutants selected on non-susceptible S. aureus strains. The wild type phage was shown to lyse up to 65%, whereas host-range mutants up to 95% of S. aureus strains under study. Genomic DNAs of five phages were sequenced using 454 Genome Sequencer Junior (Roche). Bacteriophage DNA sequences were assembled by software GS De novo Assembler and GS Reference Mapper (Roche). Genome of phage K was chosen as the reference.
dcterms:title
Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses
skos:prefLabel
Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses
skos:notation
RIV/00216224:14310/14:00073314!RIV15-MSM-14310___
n3:aktivita
n4:P
n3:aktivity
P(EE2.4.31.0155), P(TA01010405)
n3:cisloPeriodika
S1
n3:dodaniDat
n14:2015
n3:domaciTvurceVysledku
n10:9812067 n10:2074052 n10:3948005 n10:3440656 n10:5051207
n3:druhVysledku
n17:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
6944
n3:idVysledku
RIV/00216224:14310/14:00073314
n3:jazykVysledku
n12:eng
n3:klicovaSlova
bacteriophage therapy; Staphylococcus aureus; Twortlikevirus
n3:klicoveSlovo
n8:bacteriophage%20therapy n8:Staphylococcus%20aureus n8:Twortlikevirus
n3:kodStatuVydavatele
RU - Ruská federace
n3:kontrolniKodProRIV
[51E4C02E7A7E]
n3:nazevZdroje
Russian Journal of Infection and Immunity
n3:obor
n5:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
7
n3:projekt
n7:TA01010405 n7:EE2.4.31.0155
n3:rokUplatneniVysledku
n14:2014
n3:svazekPeriodika
4
n3:tvurceVysledku
Doškař, Jiří Varga, Marian Benešík, Martin Mašlaňová, Ivana Boštík, Josef Moša, Marek Pantůček, Roman
s:issn
2220-7619
s:numberOfPages
2
n18:organizacniJednotka
14310