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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F14%3A00073256%21RIV15-MZE-14310___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx. Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx.
dcterms:title
Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity
skos:prefLabel
Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity
skos:notation
RIV/00216224:14310/14:00073256!RIV15-MZE-14310___
n5:aktivita
n6:P
n5:aktivity
P(QJ1210047)
n5:dodaniDat
n10:2015
n5:domaciTvurceVysledku
n7:2304155 n7:1942859 n7:9414991
n5:druhVysledku
n15:O
n5:duvernostUdaju
n13:S
n5:entitaPredkladatele
n17:predkladatel
n5:idSjednocenehoVysledku
21270
n5:idVysledku
RIV/00216224:14310/14:00073256
n5:jazykVysledku
n14:eng
n5:klicovaSlova
entomopathogenic nematodes; honeaybee; Photorhabdus luminescens; Heterorhabditis bacteriophora
n5:klicoveSlovo
n9:Heterorhabditis%20bacteriophora n9:honeaybee n9:entomopathogenic%20nematodes n9:Photorhabdus%20luminescens
n5:kontrolniKodProRIV
[8A2089D4B824]
n5:obor
n16:EC
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
4
n5:projekt
n11:QJ1210047
n5:rokUplatneniVysledku
n10:2014
n5:tvurceVysledku
Hyršl, Pavel Berka, Jakub Dobeš, Pavel Vojtek, Libor
n12:organizacniJednotka
14310