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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F14%3A00073254%21RIV15-MSM-14310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Phagocytosis is one of the most important innate immunity mechanisms which prevents organism against pathogens overcoming the natural barriers. There are several methods widely used for evaluation of phagocytosis efficiency. One of them is to measure the level of reactive oxygen species (ROS) produced by phagocytes - respiratory burst of phagocytes. ROS production can be elicited by addition of activators such as lipopolysaccharide (LPS), zymosan, starch particles, phorbol-12-miristate-13-acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanin (fMLP). Produced ROS cause oxidation of luminophore which subsequently emits the energy in form of light measured by luminometers. Luminol is the most widely used luminophore. On the other hand evaluation of respiratory burst using this method on birds or insect samples is not possible due to a low production of ROS and low sensitivity of luminol. Phagocytosis is one of the most important innate immunity mechanisms which prevents organism against pathogens overcoming the natural barriers. There are several methods widely used for evaluation of phagocytosis efficiency. One of them is to measure the level of reactive oxygen species (ROS) produced by phagocytes - respiratory burst of phagocytes. ROS production can be elicited by addition of activators such as lipopolysaccharide (LPS), zymosan, starch particles, phorbol-12-miristate-13-acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanin (fMLP). Produced ROS cause oxidation of luminophore which subsequently emits the energy in form of light measured by luminometers. Luminol is the most widely used luminophore. On the other hand evaluation of respiratory burst using this method on birds or insect samples is not possible due to a low production of ROS and low sensitivity of luminol.
dcterms:title
Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes
skos:prefLabel
Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes
skos:notation
RIV/00216224:14310/14:00073254!RIV15-MSM-14310___
n3:aktivita
n11:I n11:P
n3:aktivity
I, P(QJ1210047)
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n5:7033265 n5:9414991 n5:1942859 n5:2304155
n3:druhVysledku
n9:O
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
7108
n3:idVysledku
RIV/00216224:14310/14:00073254
n3:jazykVysledku
n4:eng
n3:klicovaSlova
Chemiluminiscence; phagocytes; Pholasin; reactive oxygen species
n3:klicoveSlovo
n8:Pholasin n8:phagocytes n8:reactive%20oxygen%20species n8:Chemiluminiscence
n3:kontrolniKodProRIV
[171C7478E72E]
n3:obor
n16:EC
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:projekt
n14:QJ1210047
n3:rokUplatneniVysledku
n10:2014
n3:tvurceVysledku
Vinkler, Michal Prokopová, Lucie Vinklerova, Jitka Vojtek, Libor Dobeš, Pavel Hyršl, Pavel
n13:organizacniJednotka
14310