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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F14%3A00073253%21RIV15-MSM-14310___
rdf:type
skos:Concept n19:Vysledek
dcterms:description
We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement of antibacterial activity in insect haemolymph. This method is based on the production of the bioluminescence signal depending on the viability of bacterial cells. We observed a significant rapid dose-dependent decrease in bioluminescence using both bacterial species, and Bombyx mori or Galleria mellonella haemolymph, which was confirmed by the decrease in bacterial viability determined by plating. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed for haemolymph plasma without haemocytes. Antibacterial activity directed against Gram negative bacteria was recorded in unaffected insect larvae as well as after septic injury; increased antibacterial activity of haemolymph was detected in the latter case confirming the inducibility of antimicrobial agents. We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement of antibacterial activity in insect haemolymph. This method is based on the production of the bioluminescence signal depending on the viability of bacterial cells. We observed a significant rapid dose-dependent decrease in bioluminescence using both bacterial species, and Bombyx mori or Galleria mellonella haemolymph, which was confirmed by the decrease in bacterial viability determined by plating. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed for haemolymph plasma without haemocytes. Antibacterial activity directed against Gram negative bacteria was recorded in unaffected insect larvae as well as after septic injury; increased antibacterial activity of haemolymph was detected in the latter case confirming the inducibility of antimicrobial agents.
dcterms:title
Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph
skos:prefLabel
Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph
skos:notation
RIV/00216224:14310/14:00073253!RIV15-MSM-14310___
n3:aktivita
n6:P n6:I
n3:aktivity
I, P(EE2.3.30.0009), P(GP206/09/P470), P(QJ1210047)
n3:cisloPeriodika
3
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n12:2304155 n12:1942859 n12:9414991
n3:druhVysledku
n7:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
5427
n3:idVysledku
RIV/00216224:14310/14:00073253
n3:jazykVysledku
n11:eng
n3:klicovaSlova
Antibacterial activity; antimicrobial peptides; Lepidoptera; bioluminescent bacteria; Bombyx mori; Escherichia coli; Galleria mellonella; Photorhabdus luminescens
n3:klicoveSlovo
n4:Bombyx%20mori n4:bioluminescent%20bacteria n4:Photorhabdus%20luminescens n4:Galleria%20mellonella n4:Lepidoptera n4:antimicrobial%20peptides n4:Escherichia%20coli n4:Antibacterial%20activity
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[51ACC2ABEAA2]
n3:nazevZdroje
European Journal of Entomology
n3:obor
n18:EC
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n14:EE2.3.30.0009 n14:QJ1210047 n14:GP206%2F09%2FP470
n3:rokUplatneniVysledku
n10:2014
n3:svazekPeriodika
111
n3:tvurceVysledku
Büyükgüzel, Ender Dobeš, Pavel Vojtek, Libor Atosuo, Janne Hyršl, Pavel
s:issn
1210-5759
s:numberOfPages
6
n17:doi
10.14411/eje.2014.045
n13:organizacniJednotka
14310