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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F13%3A00069595%21RIV14-MSM-14310___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Early childhood caries (ECC) has become a serious medical problem worldwide in the last decade. Bacterial microflora of the dental plaque and oral cavity is considered an important factor in the formation and progression of dental caries. The aim of this study was strain typing and comparison of bacterial isolates retrieved from caries lesions and root canal contents of the same teeth. In total, 18 pairs of presumptive streptococci and lactobacilli retrieved from dental caries and root canals isolated from ECC-affected children, were selected on the basis of biotyping results and rep-PCR fingerprinting with (GTG)5 primer. Strain typing was further done using the RiboPrinter microbial characterization system (DuPont Qualicon). The automated ribotyping determined 14 pairs of the strains (77.8 %) to be identical. The results obtained confirmed that identical bacterial strains colonized both the decayed dental surface and the necrotic content of the dental pulp cavity during the cariogenesis. Early childhood caries (ECC) has become a serious medical problem worldwide in the last decade. Bacterial microflora of the dental plaque and oral cavity is considered an important factor in the formation and progression of dental caries. The aim of this study was strain typing and comparison of bacterial isolates retrieved from caries lesions and root canal contents of the same teeth. In total, 18 pairs of presumptive streptococci and lactobacilli retrieved from dental caries and root canals isolated from ECC-affected children, were selected on the basis of biotyping results and rep-PCR fingerprinting with (GTG)5 primer. Strain typing was further done using the RiboPrinter microbial characterization system (DuPont Qualicon). The automated ribotyping determined 14 pairs of the strains (77.8 %) to be identical. The results obtained confirmed that identical bacterial strains colonized both the decayed dental surface and the necrotic content of the dental pulp cavity during the cariogenesis.
dcterms:title
Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases
skos:prefLabel
Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases
skos:notation
RIV/00216224:14310/13:00069595!RIV14-MSM-14310___
n13:predkladatel
n14:orjk%3A14310
n3:aktivita
n8:P
n3:aktivity
P(EE2.3.20.0183)
n3:cisloPeriodika
6
n3:dodaniDat
n19:2014
n3:domaciTvurceVysledku
n18:9384839 n18:3787672 n18:8037051 n18:1096311
n3:druhVysledku
n4:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
73698
n3:idVysledku
RIV/00216224:14310/13:00069595
n3:jazykVysledku
n20:eng
n3:klicovaSlova
biotyping; ribotyping; rep-PCR; caries
n3:klicoveSlovo
n7:rep-PCR n7:ribotyping n7:caries n7:biotyping
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[088CB067C0F1]
n3:nazevZdroje
Folia Microbiologica
n3:obor
n16:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n9:EE2.3.20.0183
n3:rokUplatneniVysledku
n19:2013
n3:svazekPeriodika
58
n3:tvurceVysledku
Nováková, Dana Švec, Pavel Sedláček, Ivo Kukletová, Martina Žáčková, Lenka
n3:wos
000324835100029
s:issn
0015-5632
s:numberOfPages
8
n6:doi
10.1007/s12223-013-0254-6
n12:organizacniJednotka
14310