This HTML5 document contains 48 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
n20http://linked.opendata.cz/ontology/domain/vavai/riv/typAkce/
dctermshttp://purl.org/dc/terms/
n14http://purl.org/net/nknouf/ns/bibtex#
n6http://localhost/temp/predkladatel/
n19http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n18http://linked.opendata.cz/resource/domain/vavai/subjekt/
n12http://linked.opendata.cz/ontology/domain/vavai/
n17http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14310%2F13%3A00068522%21RIV14-MSM-14310___/
n5https://schema.org/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n7http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n21http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n13http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F13%3A00068522%21RIV14-MSM-14310___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
We focused interactions of zinc ions and DNA in this study. Interactions were monitored using UV/VIS spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified fragment of DNA of 498 bp. DNA was subsequently incubated with Zn(II) ions for 60 min at 25 C. After incubation, samples were dialyzed and analysed immediately. In this way, DNA was converted into a metal bound DNA (M-DNA). Interaction of Zn(II) with DNA showed a change of the absorption spectrum (190-350 nm) and a decrease in the M-DNA melting temperature. Spectrophotometric (UV/VIS) analysis showed that increasing concentrations of Zn(II) led to an increase in absorbance at 200 nm and a decrease in absorbance at 260 nm. Application of 5.5 microM Zn(II) caused a decrease in the melting temperature (Tm) for about 7.5 C in average in comparison with control (75.5 +- 3 C). The lowest melting temperature (60.5 +- 2.5 C) was observed after application of 33 microM Zn(II). We focused interactions of zinc ions and DNA in this study. Interactions were monitored using UV/VIS spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified fragment of DNA of 498 bp. DNA was subsequently incubated with Zn(II) ions for 60 min at 25 C. After incubation, samples were dialyzed and analysed immediately. In this way, DNA was converted into a metal bound DNA (M-DNA). Interaction of Zn(II) with DNA showed a change of the absorption spectrum (190-350 nm) and a decrease in the M-DNA melting temperature. Spectrophotometric (UV/VIS) analysis showed that increasing concentrations of Zn(II) led to an increase in absorbance at 200 nm and a decrease in absorbance at 260 nm. Application of 5.5 microM Zn(II) caused a decrease in the melting temperature (Tm) for about 7.5 C in average in comparison with control (75.5 +- 3 C). The lowest melting temperature (60.5 +- 2.5 C) was observed after application of 33 microM Zn(II).
dcterms:title
BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA
skos:prefLabel
BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA
skos:notation
RIV/00216224:14310/13:00068522!RIV14-MSM-14310___
n12:predkladatel
n18:orjk%3A14310
n3:aktivita
n21:I
n3:aktivity
I
n3:dodaniDat
n13:2014
n3:domaciTvurceVysledku
n19:3790185
n3:druhVysledku
n10:D
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
63535
n3:idVysledku
RIV/00216224:14310/13:00068522
n3:jazykVysledku
n11:eng
n3:klicovaSlova
DNA; zinc; UV/VIS spectrophotometry
n3:klicoveSlovo
n7:UV%2FVIS%20spectrophotometry n7:zinc n7:DNA
n3:kontrolniKodProRIV
[1F4A3D062B11]
n3:mistoKonaniAkce
Brno
n3:mistoVydani
Brno
n3:nazevZdroje
XIII. Pracovní setkání fyzikálních chemiků a elektrochemiků, sborník
n3:obor
n9:CF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
10
n3:rokUplatneniVysledku
n13:2013
n3:tvurceVysledku
Nejdl, Lukáš Kudr, Jiří Adam, Vojtěch Trnková, Libuše Kizek, René Kopel, Pavel Zehnálek, Josef Ruttkay-Nedecký, Branislav Šmerková, Kristýna Dostálová, Simona
n3:typAkce
n20:EUR
n3:zahajeniAkce
2013-01-01+01:00
s:numberOfPages
2
n14:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně
n5:isbn
9788073757571
n6:organizacniJednotka
14310