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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F13%3A00066817%21RIV14-MSM-14310___
rdf:type
n17:Vysledek skos:Concept
dcterms:description
Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide, yet serious gaps exist in understanding their complex toxic effects, including the role of lipopolysaccharides (LPS). The present comparative study focused on the levels and biological activities of LPS isolated from Microcystis aeruginosa, which is one of the most globally distributed toxic species. Using hot phenol extraction, LPS was isolated from 3 laboratory cultures and 11 natural water blooms. It formed 0.2-0.7% of the original dry biomass of the cyanobacteria, based on gravimetry. Additional analyses by commercial anti-LPS ELISA were correlated with gravimetry but showed concentrations that were about 7-times lower, which indicated either impurities in isolated LPS or the poor cross-reactivity of the antibodies used. LPS isolates from M. Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide, yet serious gaps exist in understanding their complex toxic effects, including the role of lipopolysaccharides (LPS). The present comparative study focused on the levels and biological activities of LPS isolated from Microcystis aeruginosa, which is one of the most globally distributed toxic species. Using hot phenol extraction, LPS was isolated from 3 laboratory cultures and 11 natural water blooms. It formed 0.2-0.7% of the original dry biomass of the cyanobacteria, based on gravimetry. Additional analyses by commercial anti-LPS ELISA were correlated with gravimetry but showed concentrations that were about 7-times lower, which indicated either impurities in isolated LPS or the poor cross-reactivity of the antibodies used. LPS isolates from M.
dcterms:title
The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria
skos:prefLabel
The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria
skos:notation
RIV/00216224:14310/13:00066817!RIV14-MSM-14310___
n17:predkladatel
n18:orjk%3A14310
n3:aktivita
n4:P n4:I
n3:aktivity
I, P(ED0001/01/01), P(GP13-27695P)
n3:cisloPeriodika
2013
n3:dodaniDat
n5:2014
n3:domaciTvurceVysledku
n8:2554364 n8:5005582 n8:4982800 n8:7943261
n3:druhVysledku
n10:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
81315
n3:idVysledku
RIV/00216224:14310/13:00066817
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Endotoxin; Cyanobacteria; Water bloom; Lipopolysaccharide; Microcystis
n3:klicoveSlovo
n13:Lipopolysaccharide n13:Water%20bloom n13:Microcystis n13:Endotoxin n13:Cyanobacteria
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[48B58DD4B6CE]
n3:nazevZdroje
Toxicon
n3:obor
n6:DJ
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:projekt
n7:GP13-27695P n7:ED0001%2F01%2F01
n3:rokUplatneniVysledku
n5:2013
n3:svazekPeriodika
76
n3:tvurceVysledku
Sindlerova Svihalkova, Lenka Kubala, Lukáš Adamovský, Ondřej Bláha, Luděk Bláhová, Lucie Zounková, Radka
n3:wos
000328658600023
s:issn
0041-0101
s:numberOfPages
10
n12:doi
10.1016/j.toxicon.2013.10.011
n16:organizacniJednotka
14310