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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F13%3A00066576%21RIV14-MSM-14310___
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Amino acids (AA) have no native fluorescence thus chemical modification (so called derivatization) of AA has to be performed prior the detection by a fluorescent detector. There is a lot of derivatization reagents commercially available differing in reactivity, excitation and emission wavelengths, quantum yield, product stability etc. Naphtalene-2,3-dicarbaldehyde (NDA) is a non-fluorescent reagent giving fluorescent product of primary amines. Derivatization procedure is quite simple, however has to be optimized to minimize undesired by-products of benzoin condensation. The by-products can exhibit fluorescence or form crystals which can clog capillary. The novel derivatization protocol using NDA for AA derivatization is subject of this contribution. Amino acids (AA) have no native fluorescence thus chemical modification (so called derivatization) of AA has to be performed prior the detection by a fluorescent detector. There is a lot of derivatization reagents commercially available differing in reactivity, excitation and emission wavelengths, quantum yield, product stability etc. Naphtalene-2,3-dicarbaldehyde (NDA) is a non-fluorescent reagent giving fluorescent product of primary amines. Derivatization procedure is quite simple, however has to be optimized to minimize undesired by-products of benzoin condensation. The by-products can exhibit fluorescence or form crystals which can clog capillary. The novel derivatization protocol using NDA for AA derivatization is subject of this contribution.
dcterms:title
Naphthalene-2,3-dicarbaldehyde derivatization of amino acids - an improved technique for minimization of benzoin condensation Naphthalene-2,3-dicarbaldehyde derivatization of amino acids - an improved technique for minimization of benzoin condensation
skos:prefLabel
Naphthalene-2,3-dicarbaldehyde derivatization of amino acids - an improved technique for minimization of benzoin condensation Naphthalene-2,3-dicarbaldehyde derivatization of amino acids - an improved technique for minimization of benzoin condensation
skos:notation
RIV/00216224:14310/13:00066576!RIV14-MSM-14310___
n13:predkladatel
n14:orjk%3A14310
n3:aktivita
n15:S n15:P
n3:aktivity
P(EE2.3.20.0182), P(GAP206/11/0009), S
n3:cisloPeriodika
S3
n3:dodaniDat
n12:2014
n3:domaciTvurceVysledku
n4:4767187 n4:7201761 n4:7118317
n3:druhVysledku
n17:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
90647
n3:idVysledku
RIV/00216224:14310/13:00066576
n3:jazykVysledku
n19:eng
n3:klicovaSlova
capillary electrophoresis; LIF; Amino acids
n3:klicoveSlovo
n9:Amino%20acids n9:capillary%20electrophoresis n9:LIF
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[48B21DA0B02C]
n3:nazevZdroje
Chemické listy
n3:obor
n18:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n6:EE2.3.20.0182 n6:GAP206%2F11%2F0009
n3:rokUplatneniVysledku
n12:2013
n3:svazekPeriodika
107
n3:tvurceVysledku
Mádr, Aleš Celá, Andrea Dědová, Tereza Glatz, Zdeněk
n3:wos
000328730800028
s:issn
0009-2770
s:numberOfPages
2
n5:organizacniJednotka
14310