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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F13%3A00066570%21RIV14-MSM-14310___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Haloalkane dehalogenases (EC 3.8.1.5) are alfa/beta-hydrolases cleaving a carbon-halogen bond in more than hundred halogenated aliphatic and cyclic compounds. Due to interesting chemistry, broad substrate specificity and high robustness, the members of this enzyme family have been employed in several practical applications: (i) biocatalytic preparation of optically pure building-blocks for organic synthesis, (ii) recycling of by-products from chemical processes, (iii) bioremediation of toxic environmental pollutants, (iv) decontamination of warfare agents, (v) biosensing of environmental pollutants, and (vi) protein tagging for cell imaging and protein analysis. The review discusses these applications in the context of biochemical properties of individual enzymes, when nine out of seventeen characterized enzymes possess properties suitable for practical applications. Haloalkane dehalogenases (EC 3.8.1.5) are alfa/beta-hydrolases cleaving a carbon-halogen bond in more than hundred halogenated aliphatic and cyclic compounds. Due to interesting chemistry, broad substrate specificity and high robustness, the members of this enzyme family have been employed in several practical applications: (i) biocatalytic preparation of optically pure building-blocks for organic synthesis, (ii) recycling of by-products from chemical processes, (iii) bioremediation of toxic environmental pollutants, (iv) decontamination of warfare agents, (v) biosensing of environmental pollutants, and (vi) protein tagging for cell imaging and protein analysis. The review discusses these applications in the context of biochemical properties of individual enzymes, when nine out of seventeen characterized enzymes possess properties suitable for practical applications.
dcterms:title
Haloalkane dehalogenases: Biotechnological applications Haloalkane dehalogenases: Biotechnological applications
skos:prefLabel
Haloalkane dehalogenases: Biotechnological applications Haloalkane dehalogenases: Biotechnological applications
skos:notation
RIV/00216224:14310/13:00066570!RIV14-MSM-14310___
n8:predkladatel
n15:orjk%3A14310
n4:aktivita
n20:P
n4:aktivity
P(ED0001/01/01), P(GA203/08/0114), P(GAP207/12/0775), P(GAP503/12/0572), P(LG13010)
n4:cisloPeriodika
1
n4:dodaniDat
n12:2014
n4:domaciTvurceVysledku
n5:2475960 n5:1030175 n5:1826050 n5:1344846 n5:2070103 n5:7612656
n4:druhVysledku
n19:J
n4:duvernostUdaju
n7:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
77011
n4:idVysledku
RIV/00216224:14310/13:00066570
n4:jazykVysledku
n17:eng
n4:klicovaSlova
Biocatalysis; Biodegradation; Biosensors; Cell imaging; Protein analysis
n4:klicoveSlovo
n16:Cell%20imaging n16:Biodegradation n16:Protein%20analysis n16:Biocatalysis n16:Biosensors
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[2D06AD6AE589]
n4:nazevZdroje
Biotechnology Journal
n4:obor
n11:CE
n4:pocetDomacichTvurcuVysledku
6
n4:pocetTvurcuVysledku
7
n4:projekt
n6:GA203%2F08%2F0114 n6:GAP207%2F12%2F0775 n6:LG13010 n6:ED0001%2F01%2F01 n6:GAP503%2F12%2F0572
n4:rokUplatneniVysledku
n12:2013
n4:svazekPeriodika
8
n4:tvurceVysledku
Bidmanová, Šárka Dvořák, Pavel Pavelka, Antonín Prokop, Zbyněk Koudeláková, Táňa Chaloupková, Radka Damborský, Jiří
n4:wos
000312989500012
s:issn
1860-6768
s:numberOfPages
15
n10:doi
10.1002/biot.201100486
n9:organizacniJednotka
14310