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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F12%3A00060129%21RIV13-MSM-14310___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Electrochemical study of Beta-sheet breaker prion protein is the main aim of this study. For this purpose, cyclic voltammetry (CV), differential pulse voltammetry, differential pulse voltammetry Brdicka reaction and chronopotentiometric stripping analysis were used. Under the optimal conditions (phosphate buffer, pH 7.38 and time of accumulation 100 s), detected by DPV prion was characterized using different techniques and their limits of detection were found. Adsorptive transfer stripping technique coupled with the abovementioned methods offers very lower detection limits. The lowest limits of detection were determined by CPSA AdTS i.e. 25 pmol in volume of 5 microl. CPSA is therefore a very sensitive tool for the studying of prion behaviour. Moreover, the influence of heat denaturation was observed. It clearly follows from the results obtained that signals of prion decreased linearly depending on the duration of the heat treatment at 99 C for various time intervals: 0, 15, 30, 45, and 60 min. Electrochemical study of Beta-sheet breaker prion protein is the main aim of this study. For this purpose, cyclic voltammetry (CV), differential pulse voltammetry, differential pulse voltammetry Brdicka reaction and chronopotentiometric stripping analysis were used. Under the optimal conditions (phosphate buffer, pH 7.38 and time of accumulation 100 s), detected by DPV prion was characterized using different techniques and their limits of detection were found. Adsorptive transfer stripping technique coupled with the abovementioned methods offers very lower detection limits. The lowest limits of detection were determined by CPSA AdTS i.e. 25 pmol in volume of 5 microl. CPSA is therefore a very sensitive tool for the studying of prion behaviour. Moreover, the influence of heat denaturation was observed. It clearly follows from the results obtained that signals of prion decreased linearly depending on the duration of the heat treatment at 99 C for various time intervals: 0, 15, 30, 45, and 60 min.
dcterms:title
INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE
skos:prefLabel
INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE
skos:notation
RIV/00216224:14310/12:00060129!RIV13-MSM-14310___
n8:predkladatel
n12:orjk%3A14310
n3:aktivita
n6:I
n3:aktivity
I
n3:dodaniDat
n4:2013
n3:domaciTvurceVysledku
n13:3790185
n3:druhVysledku
n20:D
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
141434
n3:idVysledku
RIV/00216224:14310/12:00060129
n3:jazykVysledku
n17:eng
n3:klicovaSlova
prion protein; cyclic voltammetry; differential pulse voltammetry; Brdicka reaction; chronopotentiometry
n3:klicoveSlovo
n7:Brdicka%20reaction n7:prion%20protein n7:cyclic%20voltammetry n7:chronopotentiometry n7:differential%20pulse%20voltammetry
n3:kontrolniKodProRIV
[F575080D096F]
n3:mistoKonaniAkce
Brno
n3:mistoVydani
Brno
n3:nazevZdroje
XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků.
n3:obor
n18:CE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n4:2012
n3:tvurceVysledku
Ryvolová, Markéta Kizek, René Hubálek, Jaromír Trnková, Libuše Adam, Vojtěch Šobrová, Pavlína Hynek, David
n3:typAkce
n21:EUR
n3:zahajeniAkce
2012-01-01+01:00
s:numberOfPages
3
n16:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně
n14:isbn
9788073756185
n19:organizacniJednotka
14310