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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F12%3A00059725%21RIV13-MSM-14310___
rdf:type
skos:Concept n8:Vysledek
dcterms:description
The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5a in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions. The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5a in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.
dcterms:title
Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols
skos:prefLabel
Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols
skos:notation
RIV/00216224:14310/12:00059725!RIV13-MSM-14310___
n8:predkladatel
n9:orjk%3A14310
n3:aktivita
n15:Z
n3:aktivity
Z(MSM0021622413)
n3:cisloPeriodika
3
n3:dodaniDat
n17:2013
n3:domaciTvurceVysledku
n13:8272433 n13:6669719 n13:2240866 n13:3133745 n13:8383456
n3:druhVysledku
n16:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
133872
n3:idVysledku
RIV/00216224:14310/12:00059725
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Bioagents; Sandwich immunoassay; Biosensors; Remote sensing; Aerosol chamber
n3:klicoveSlovo
n12:Sandwich%20immunoassay n12:Aerosol%20chamber n12:Bioagents n12:Remote%20sensing n12:Biosensors
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[509BD6BED4AC]
n3:nazevZdroje
Electroanalysis
n3:obor
n6:CE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n17:2012
n3:svazekPeriodika
24
n3:tvurceVysledku
Švábenská, Eva Gustafson, Inga Šišková, Petra Žeravík, Jiří Přibyl, Jan Tjarnhage, Torbjorn Skládal, Petr
n3:wos
000300931000011
n3:zamer
n4:MSM0021622413
s:issn
1040-0397
s:numberOfPages
8
n11:doi
10.1002/elan.201100448
n18:organizacniJednotka
14310